• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

意大利中部狍和黇鹿体内戊型肝炎病毒的分子与病理学检测

Molecular and Pathological Detection of Hepatitis E Virus in Roe Deer () and Fallow Deer () in Central Italy.

作者信息

Fonti Niccolò, Pacini Maria Irene, Forzan Mario, Parisi Francesca, Periccioli Marcello, Mazzei Maurizio, Poli Alessandro

机构信息

Dipartimento di Scienze Veterinarie, Università di Pisa, Viale delle Piagge, 2-56124 Pisa, Italy.

Unità Funzionale di Sanità Pubblica Veterinaria e Sicurezza Alimentare Zona Distretto Grossetana, Dipartimento di Prevenzione, Azienda USL Toscana Sud Est, Amiata Grossetana e Colline Metallifere, Viale Cimabue, 109-58100 Grosseto, Italy.

出版信息

Vet Sci. 2022 Feb 24;9(3):100. doi: 10.3390/vetsci9030100.

DOI:10.3390/vetsci9030100
PMID:35324829
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8950858/
Abstract

Hepatitis E virus (HEV) is a common causative agent of acute hepatitis in the world, with a serious public health burden in both developing and industrialized countries. Cervids, along with wild boars and lagomorphs, are the main wild hosts of HEV in Europe and constitute a documented source of infection for humans. The aim of this study was to evaluate the presence of HEV in roe deer () and fallow deer () living in Tuscany, Central Italy. Liver samples from 48 roe deer and 60 fallow deer were collected from carcasses during the hunting seasons. Following the results obtained from molecular and histopathologic studies, 5/48 (10.4%) roe deer and 1/60 (1.7%) fallow deer liver samples were positive for the presence of HEV RNA. All PCR-positive livers were also IHC-positive for viral antigen presence, associated with degenerative and inflammatory lesions with predominantly CD3+ cellular infiltrates. This study represents the first identification in Italy of HEV RNA in roe and fallow deer and the first study in literature describing liver alterations associated with HEV infection in cervids. These results demonstrate that HEV is present in wild cervid populations in Italy and confirm the potential zoonotic role of these species.

摘要

戊型肝炎病毒（HEV）是全球急性肝炎的常见病原体，在发展中国家和工业化国家均造成严重的公共卫生负担。在欧洲，鹿类与野猪和兔形目动物一样，是戊型肝炎病毒的主要野生宿主，也是有记录的人类感染源。本研究旨在评估生活在意大利中部托斯卡纳地区的狍和黇鹿体内戊型肝炎病毒的存在情况。在狩猎季节从鹿的尸体上采集了48份狍和60份黇鹿的肝脏样本。根据分子和组织病理学研究结果，5/48（10.4%）份狍肝脏样本和1/60（1.7%）份黇鹿肝脏样本的戊型肝炎病毒RNA检测呈阳性。所有PCR阳性的肝脏在免疫组化检测中也呈病毒抗原阳性，伴有以CD3+细胞浸润为主的变性和炎症性病变。本研究是意大利首次在狍和黇鹿中鉴定出戊型肝炎病毒RNA，也是文献中首次描述鹿类动物中与戊型肝炎病毒感染相关的肝脏病变的研究。这些结果表明，戊型肝炎病毒存在于意大利的野生鹿类种群中，并证实了这些物种的潜在人畜共患病作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae6/8950858/4b18bfc2f878/vetsci-09-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae6/8950858/073a153c48b8/vetsci-09-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae6/8950858/ec4a8368e697/vetsci-09-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae6/8950858/4b18bfc2f878/vetsci-09-00100-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae6/8950858/073a153c48b8/vetsci-09-00100-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae6/8950858/ec4a8368e697/vetsci-09-00100-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dae6/8950858/4b18bfc2f878/vetsci-09-00100-g003.jpg

相似文献

1
Molecular and Pathological Detection of Hepatitis E Virus in Roe Deer () and Fallow Deer () in Central Italy.意大利中部狍和黇鹿体内戊型肝炎病毒的分子与病理学检测
Vet Sci. 2022 Feb 24;9(3):100. doi: 10.3390/vetsci9030100.
2
Shiga toxin-producing Escherichia coli isolates from red deer (Cervus elaphus), roe deer (Capreolus capreolus) and fallow deer (Dama dama) in Poland.波兰红鹿（Cervus elaphus）、狍（Capreolus capreolus）和黇鹿（Dama dama）中产志贺毒素大肠杆菌分离株。
Food Microbiol. 2020 Apr;86:103352. doi: 10.1016/j.fm.2019.103352. Epub 2019 Oct 22.
3
Serologic and Molecular Survey of Hepatitis E Virus in German Deer Populations.德国鹿群中戊型肝炎病毒的血清学和分子学调查
J Wildl Dis. 2016 Jan;52(1):106-13. doi: 10.7589/2014-12-282. Epub 2015 Nov 3.
4
and Species of Sympatric Roe Deer (), Fallow Deer (), Sika Deer () and Red Deer () in Germany.以及德国同域分布的狍（）、黇鹿（）、梅花鹿（）和马鹿（）的物种。
Pathogens. 2020 Nov 20;9(11):968. doi: 10.3390/pathogens9110968.
5
Anaplasma phagocytophilum and Babesia spp. in roe deer (Capreolus capreolus), fallow deer (Dama dama) and mouflon (Ovis musimon) in Germany.德国狍（Capreolus capreolus）、黇鹿（Dama dama）和摩弗伦羊（Ovis musimon）体内的嗜吞噬细胞无形体和巴贝斯虫属。
Mol Cell Probes. 2017 Feb;31:46-54. doi: 10.1016/j.mcp.2016.08.008. Epub 2016 Aug 19.
6
Seroprevalence of Hepatitis E Virus in Moose (), Reindeer (), Red Deer (), Roe Deer (), and Muskoxen () from Norway.挪威驼鹿（）、驯鹿（）、马鹿（）、狍（）和麝香牛（）中戊型肝炎病毒的血清流行率。
Viruses. 2021 Feb 1;13(2):224. doi: 10.3390/v13020224.
7
Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids.瑞典半家养和自由放养鹿体内网尾线虫属（毛圆科：网尾科）的分子鉴定及流行情况
J Wildl Dis. 2002 Oct;38(4):769-75. doi: 10.7589/0090-3558-38.4.769.
8
Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene.利用聚合酶链反应靶向线粒体12S rRNA基因的特定序列鉴定马鹿（Cervus elaphus）、黇鹿（Dama dama）和狍（Capreolus capreolus）的肉。
Meat Sci. 2007 Jun;76(2):234-40. doi: 10.1016/j.meatsci.2006.11.004. Epub 2007 Jan 3.
9
Hepatitis E virus antibody prevalence in wildlife in Poland.波兰野生动物中戊型肝炎病毒抗体的流行情况。
Zoonoses Public Health. 2015 Mar;62(2):105-10. doi: 10.1111/zph.12113. Epub 2014 Mar 21.
10
Real-time PCR for detection and quantification of red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) in meat mixtures.用于检测和定量肉混合物中马鹿（Cervus elaphus）、黇鹿（Dama dama）和狍（Capreolus capreolus）的实时聚合酶链反应
Meat Sci. 2008 Jun;79(2):289-98. doi: 10.1016/j.meatsci.2007.09.013. Epub 2007 Oct 5.

引用本文的文献

1
Molecular Detection and Genetic Typing of Hepatitis E Virus in Wild Animals from Slovakia.斯洛伐克野生动物中戊型肝炎病毒的分子检测与基因分型
Food Environ Virol. 2025 Aug 11;17(3):42. doi: 10.1007/s12560-025-09657-z.
2
Recent Hepatitis E Virus Infection in Wild Boars and Other Ungulates in Japan.日本野猪及其他有蹄类动物近期感染戊型肝炎病毒情况。
Viruses. 2025 Apr 4;17(4):524. doi: 10.3390/v17040524.
3
Hepatitis E Virus in the Role of an Emerging Food-Borne Pathogen.戊型肝炎病毒作为一种新兴食源性病原体所起的作用

本文引用的文献

1
Analysis of seroprevalence data on Hepatitis E virus and Toxoplasma gondii in wild ungulates for the assessment of human exposure to zoonotic meat-borne pathogens.分析野生有蹄类动物的戊型肝炎病毒和弓形虫血清流行率数据，以评估人类接触动物源食源性致病菌的情况。
Food Microbiol. 2022 Feb;101:103890. doi: 10.1016/j.fm.2021.103890. Epub 2021 Aug 24.
2
Hepatitis E Virus RNA Presence in Wild Boar Carcasses at Slaughterhouses in Italy.意大利屠宰场野猪尸体中戊型肝炎病毒RNA的存在情况
Animals (Basel). 2021 May 31;11(6):1624. doi: 10.3390/ani11061624.
3
Mechanism of Cross-Species Transmission, Adaptive Evolution and Pathogenesis of Hepatitis E Virus.
Microorganisms. 2025 Apr 12;13(4):885. doi: 10.3390/microorganisms13040885.
4
Longitudinal serological and virological survey of hepatitis E virus in wild boar (, Maremman wild boar) and fallow deer () populations in a protected area of Central Italy.意大利中部某保护区野猪（马雷马野猪）和黇鹿种群中戊型肝炎病毒的纵向血清学和病毒学调查
Front Vet Sci. 2024 Nov 27;11:1511823. doi: 10.3389/fvets.2024.1511823. eCollection 2024.
5
Serological survey on Hepatitis E virus in Namibian dogs, cats, horses, and donkeys.纳米比亚犬、猫、马和驴的戊型肝炎病毒血清学调查。
Front Vet Sci. 2024 Jul 18;11:1422001. doi: 10.3389/fvets.2024.1422001. eCollection 2024.
6
Hepatitis E Virus in Domestic Ruminants and Virus Excretion in Milk-A Potential Source of Zoonotic HEV Infection.戊型肝炎病毒在 domestic ruminants（反刍动物）和牛奶中的排泄——人畜共患戊型肝炎感染的潜在来源。
Viruses. 2024 Apr 26;16(5):684. doi: 10.3390/v16050684.
7
Detection and Characterization of Zoonotic Pathogens in Game Meat Hunted in Northwestern Italy.意大利西北部猎获的野味中人畜共患病原体的检测与特征分析。
Animals (Basel). 2024 Feb 7;14(4):562. doi: 10.3390/ani14040562.
8
A multispecies competitive nanobody-based ELISA for the detection of antibodies against hepatitis E virus.一种基于多物种竞争纳米体的 ELISA 检测方法，用于检测抗戊型肝炎病毒的抗体。
Sci Rep. 2023 Sep 18;13(1):15448. doi: 10.1038/s41598-023-41955-z.
9
Hepatitis A Virus and Hepatitis E Virus as Food- and Waterborne Pathogens-Transmission Routes and Methods for Detection in Food.甲型肝炎病毒和戊型肝炎病毒作为食源性和水源性病原体 - 食品中的传播途径和检测方法。
Viruses. 2023 Aug 12;15(8):1725. doi: 10.3390/v15081725.
10
Epidemiology of Wildlife Infectious Diseases.野生动物传染病流行病学
Vet Sci. 2023 May 5;10(5):332. doi: 10.3390/vetsci10050332.
戊型肝炎病毒的跨种传播、适应性进化和发病机制。
Viruses. 2021 May 14;13(5):909. doi: 10.3390/v13050909.
4
Hepatitis E Virus in the Food of Animal Origin: A Review.动物源食品中的戊型肝炎病毒：综述。
Foodborne Pathog Dis. 2021 Jun;18(6):368-377. doi: 10.1089/fpd.2020.2896. Epub 2021 Mar 30.
5
Hepatitis E Virus (HEV) Spread and Genetic Diversity in Game Animals in Northern Italy.意大利北部游戏动物中戊型肝炎病毒（HEV）的传播和遗传多样性。
Food Environ Virol. 2021 Jun;13(2):146-153. doi: 10.1007/s12560-021-09467-z. Epub 2021 Feb 25.
6
Classification of the Zoonotic Hepatitis E Virus Genotype 3 Into Distinct Subgenotypes.人畜共患戊型肝炎病毒3型向不同亚基因型的分类。
Front Microbiol. 2021 Jan 28;11:634430. doi: 10.3389/fmicb.2020.634430. eCollection 2020.
7
Seroprevalence of Hepatitis E Virus in Moose (), Reindeer (), Red Deer (), Roe Deer (), and Muskoxen () from Norway.挪威驼鹿（）、驯鹿（）、马鹿（）、狍（）和麝香牛（）中戊型肝炎病毒的血清流行率。
Viruses. 2021 Feb 1;13(2):224. doi: 10.3390/v13020224.
8
Surveillance Study of Hepatitis E Virus (HEV) in Domestic and Wild Ruminants in Northwestern Italy.意大利西北部家养和野生反刍动物中戊型肝炎病毒（HEV）的监测研究
Animals (Basel). 2020 Dec 9;10(12):2351. doi: 10.3390/ani10122351.
9
Seroprevalence of hepatitis E virus in dogs in Switzerland.瑞士犬类戊型肝炎病毒血清流行率
Zoonoses Public Health. 2021 Feb;68(1):8-11. doi: 10.1111/zph.12779. Epub 2020 Nov 15.
10
Hepatitis E virus (HEV) genotype 3 diversity: Identification of a novel HEV subtype in wild boar in Central Italy.戊型肝炎病毒（HEV）基因型 3 的多样性：在意大利中部野猪中发现新型 HEV 亚型。
Transbound Emerg Dis. 2021 Jul;68(4):2121-2129. doi: 10.1111/tbed.13860. Epub 2020 Oct 20.