Institute of Forensic Medicine, Faculty of Medicine, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, SI-1000 Ljubljana, Slovenia.
Int J Mol Sci. 2022 Mar 15;23(6):3170. doi: 10.3390/ijms23063170.
Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.
尸体供体组织是分离具有间充质干细胞（MSC）样特性的原代细胞的宝贵替代来源。然而，从不同组织和不同死后时间获得的原代细胞的特性尚未得到充分认识。在这里，我们旨在确定从三个膝关节和膝关节周围组织中分离具有 MSC 样特性的原代细胞的最佳组织来源，并定义死后时间对这些细胞特性的影响。我们从 32 名供体的软骨下骨髓、滑膜和骨膜中采集组织，并在不同的死后时间进行分析。使用详细的体外分析评估原代细胞，包括集落形成、三系分化、免疫表型和骨骼干细胞标志物基因表达谱分析。这些数据表明，从这三种组织中分离出来的具有 MSC 样特性的原代细胞在性质上没有差异，只是骨髓细胞中 的表达更高。原代细胞分离的成功率取决于死后时间。然而，死后 48 小时以上分离的滑膜和骨膜细胞具有更好的成骨和成软骨潜力。本研究表明，即使在死后 3 天，供体的膝关节和膝关节周围组织也是再生程序中 MSC 的战略来源。
