Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong.
State Key Lab of Chemical Biology and Drug Discovery, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong.
Emerg Microbes Infect. 2022 Dec;11(1):1049-1057. doi: 10.1080/22221751.2022.2058420.
With the development of multidrug resistance in spp. in recent years, ciprofloxacin, ceftriaxone, and azithromycin have become the principal antimicrobial agents used for the treatment of infections. The underlying mechanisms of plasmid-mediated ciprofloxacin and ceftriaxone resistance have attracted extensive research interest, but not much is focused on azithromycin resistance in . In this study, we investigated the genetic features of two conjugative plasmids and a non-transferable virulence plasmid that encode azithromycin resistance in food-borne strains. We showed that the azithromycin resistance phenotype of these strains was conferred by gene and/or the complete genetic structure ISIS Comparative genetic analysis showed that these conjugative plasmids might originate from and play a role in the rapid dissemination of azithromycin resistance in . These conjugative plasmids may also serve as a reservoir of antimicrobial resistance (AMR) genes in in which these AMR genes may be acquired by the virulence plasmids of via genetic transposition events. Importantly, the formation of a novel macrolide-resistance and virulence-encoding plasmid, namely pS1380-118 kb, was observed in this study. This plasmid was found to exhibit transmission potential and pose a serious health threat as the extensive transmission of azithromycin resistant and virulent strains would further compromise the effectiveness of treatment for salmonellosis. Further surveillance and research on the dissemination and evolution routes of pS1380-118kb-like plasmids in potential human pathogens of the family of are necessary.
近年来， spp. 的多药耐药性发展，使得环丙沙星、头孢曲松和阿奇霉素成为治疗 感染的主要抗菌药物。质粒介导的环丙沙星和头孢曲松耐药的潜在机制引起了广泛的研究兴趣，但对于 中的阿奇霉素耐药性关注较少。在本研究中，我们研究了食源性病原体中编码阿奇霉素耐药性的两个可转移质粒和一个不可转移毒力质粒的遗传特征。结果表明，这些菌株的阿奇霉素耐药表型是由 基因和/或完整的遗传结构 ISIS 编码的。比较遗传分析表明，这些可转移质粒可能起源于 ，并在 中阿奇霉素耐药性的快速传播中发挥作用。这些可转移质粒也可能成为 中抗菌药物耐药性（AMR）基因的库，这些 AMR 基因可能通过遗传转位事件被 毒力质粒获得。重要的是，在本研究中观察到了一种新型大环内酯类耐药和毒力编码质粒，即 pS1380-118kb 的形成。该质粒具有传播潜力，构成严重的健康威胁，因为广泛传播的阿奇霉素耐药和毒力 菌株将进一步降低沙门氏菌病治疗的效果。有必要对 pS1380-118kb 样质粒在 家族潜在人类病原体中的传播和进化途径进行进一步监测和研究。
