LAQV, REQUIMTE, Laboratory of Applied Chemistry, Department of Chemical Sciences, Faculty of Pharmacy, Porto University, Rua Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal.
Unidade de Investigação para o Desenvolvimento do Interior, Instituto Politécnico da Guarda, Avenida Dr. Francisco de Sá Carneiro, No 50, 6300-559 Guarda, Portugal.
Molecules. 2022 Mar 11;27(6):1825. doi: 10.3390/molecules27061825.
The aim of this study is the development of an automated method for myeloperoxidase activity evaluation and its application in testing the inhibitory action of different plant extracts on the activity of the enzyme. This enzyme has its concentration increased in inflammatory and infectious processes, so it is a possible target to limit these processes. Therefore, an automatic sequential in-jection analysis (SIA) system was optimized and demonstrated that it is possible to obtain results with satisfactory accuracy and precision. With the developed method, plant extracts were studied, as promising candidates for MPO inhibition. In the group of selected plant extracts, IC50 values from 0.029 ± 0.002 mg/mL to 35.4 ± 3.5 mg/mL were obtained. L. proved to be the most inhibitory extract for MPO based on its phenolic compound content. The coupling of an automatic SIA method to MPO inhibition assays is a good alternative to other conventional methods, due to its simplicity and speed. This work also supports the pharmacological use of these species that inhibit MPO, and exhibit activity that may be related to the treatment of infection and inflammation.
本研究旨在开发一种自动测定髓过氧化物酶（MPO）活性的方法，并将其应用于测试不同植物提取物对该酶活性的抑制作用。这种酶在炎症和感染过程中浓度增加，因此是限制这些过程的一个可能靶点。因此，我们对自动顺序注射分析（SIA）系统进行了优化，并证明该系统能够获得具有令人满意的准确性和精密度的结果。使用所开发的方法对植物提取物进行了研究，这些提取物是潜在的 MPO 抑制剂。在所选择的植物提取物组中，获得了 0.029 ± 0.002mg/mL 至 35.4 ± 3.5mg/mL 的 IC50 值。基于其酚类化合物含量，L. 提取物被证明对 MPO 具有最强的抑制作用。自动 SIA 方法与 MPO 抑制测定相结合，由于其简单和快速，是对其他常规方法的一种很好的替代方法。这项工作还支持了这些具有抑制 MPO 活性并表现出可能与治疗感染和炎症相关的活性的物种的药理学用途。
