Department of Anesthesiology and Pain Medicine, Korea University Anam Hospital, Seoul 02841, Korea.
Smith College of Liberal Arts, Sahmyook University, 815, Hwarang-ro, Nowon-gu, Seoul 01795, Korea.
Molecules. 2022 Mar 11;27(6):1837. doi: 10.3390/molecules27061837.
Staphylococcus aureus bacteria is a ubiquitous Gram-positive microorganism that causes infections related to the sudden infant death syndrome. Recently, basic detection methods depend on complicated PCR amplification, electric separation, spectric adsorption and other detection systems. However, in this study, simplified sensitive voltammetric skills are developed. To identify an effective diagnostic method for Staphylococcus aureus (SA), a voltammetric sensing probe was sought using mercury immobilized on a carbon nanotube sensor (MCN). The voltammetric MCN conditions were optimized through stripping and cyclic voltammetry. Diagnostic electrolyte was used on non-treated blood sera as an electrolyte solution. The optimum cyclic and stripping analytical working range was 0.5-4.0 mL (3 × 10~5 × 10 CFU/0.5 mL) SA. The statistic relative standard deviation of 0.1 mL SA was observed to be 0.0078 (n = 5). Using the optimum parameters, a diagnostic test was performed by the direct assay of SA in non-treated human blood and patient sera. Here, the developed results can be used for the direct assay of non-treated blood sera, organ monitoring, in-vivo diagnosis, and other assays requiring SA detection.
金黄色葡萄球菌是一种普遍存在的革兰氏阳性微生物，可引起与婴儿猝死综合征相关的感染。最近，基本检测方法依赖于复杂的 PCR 扩增、电泳分离、光谱吸附和其他检测系统。然而，在这项研究中，开发了简化的敏感伏安法技术。为了鉴定金黄色葡萄球菌（SA）的有效诊断方法，我们使用固定在碳纳米管传感器上的汞（MCN）寻求伏安传感探针。通过剥离和循环伏安法优化了伏安 MCN 条件。诊断电解质用作非处理血血清的电解质溶液。最佳循环和剥离分析工作范围为 0.5-4.0 mL（3×10~5×10 CFU/0.5 mL）SA。观察到 0.1 mL SA 的统计相对标准偏差为 0.0078（n=5）。使用最佳参数，通过非处理人血和患者血清中的 SA 直接测定进行诊断测试。在这里，开发的结果可用于非处理血血清的直接测定、器官监测、体内诊断和其他需要检测 SA 的测定。
