Department of Physics, Annamalai University, Chidambaram, 608002, TN, India.
Department of Pharmacy, Annamalai University, Chidambaram, 608002, TN, India.
Appl Biochem Biotechnol. 2023 Jun;195(6):3764-3786. doi: 10.1007/s12010-022-03866-z. Epub 2022 Mar 26.
Zinc oxide (ZnO) NPs, owing to their broad biomedical applications, have recently attracted the scientific community with incredible interest as therapeutic agents. So, the present study aims at preparation of ZnO NPs, using Tragia involucrata leaf extract and exploring their capability as antioxidant, anticancer and anti-inflammatory agents. Besides, the ointment formulation and electrochemical studies were also carried out in this work. The antioxidant activity of the synthesized ZnO NPs was evaluated using DPPH assay method and the results clearly showed higher inhibition of about 70% and lower inhibition of about 14% for 100 µg/ml and 25 µg/ml concentrations, respectively. The cytotoxic effects of ZnO NPs were evaluated against human cancer cell lines such as A549 (lungs), HeLa (cervical), HeP-2 (laryngeal) and MCF-7 (breast). The outcome of this investigation confirmed the effectiveness of the synthesized NPs against HeP-2 even at the lowest concentration. The anti-inflammatory activity was measured by the inhibition of protein denaturation assay. A higher inhibition of about 54% was noticed at the concentration of 100 µg/ml. In the case of the ointment formulation study, the pastes prepared using the biosynthesized ZnO NPs and commercially available ZnO powder were compared and evaluated using the parameters such as pH, spreadability, moisture content, extrudability, foamability and physical examinations. As it has been noticed that all the observed parameters were matching well with those of the commercially available ZnO powder, ZnO NPs, synthesized using Tragia involucrata, may be suggested for the clinical trials. Cyclic voltammetry was used to measure the specific capacitance of the synthesized ZnO NPs for different scan rates. The results of this study showed the gradual decrease in specific capacitance value for the corresponding increase in scan rates. Therefore, the results of present study indicated that ZnO NPs prepared using Tragia involucrata leaves were found to be effective for all the above chosen applications and hence, have multifunctional capacity.
氧化锌 (ZnO) NPs 由于其广泛的生物医学应用，最近作为治疗剂引起了科学界的极大兴趣。因此，本研究旨在使用 Tragia involucrata 叶提取物制备 ZnO NPs，并探索其作为抗氧化剂、抗癌剂和抗炎剂的能力。此外，本工作还进行了软膏制剂和电化学研究。通过 DPPH 法评价了合成 ZnO NPs 的抗氧化活性，结果清楚地表明，浓度为 100μg/ml 和 25μg/ml 时，抑制率分别约为 70%和约 14%。用 DPPH 法评价了 ZnO NPs 对人癌细胞系如 A549（肺）、HeLa（宫颈）、HeP-2（喉）和 MCF-7（乳腺）的细胞毒性作用。这项研究的结果证实了合成的 NPs 对 HeP-2 的有效性，即使在最低浓度下也是如此。通过蛋白质变性抑制测定法测定了抗炎活性。在 100μg/ml 的浓度下，观察到约 54%的更高抑制率。在软膏制剂研究中，比较了使用生物合成的 ZnO NPs 和市售 ZnO 粉末制备的糊剂，并使用 pH 值、铺展性、水分含量、挤出性、泡沫性和物理检查等参数进行了评估。由于注意到所有观察到的参数都与市售 ZnO 粉末的参数相匹配，因此可以建议使用 Tragia involucrata 合成的 ZnO NPs 进行临床试验。使用循环伏安法测量了不同扫描速率下合成 ZnO NPs 的比电容。研究结果表明，比电容值随扫描速率的增加而逐渐降低。因此，本研究结果表明，使用 Tragia involucrata 叶制备的 ZnO NPs 在所有上述选择的应用中都有效，因此具有多功能性。
