Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Probiotics Antimicrob Proteins. 2022 Aug;14(4):675-689. doi: 10.1007/s12602-022-09934-4. Epub 2022 Mar 29.
Difficulties in delivering antimicrobial agents to wound areas and emersion of multiple drug resistant organisms (MDROs) have converted managing burn infections into a complicated task in medicine. Probiotics emerged not only as a probable solution for burn infections but also as an accelerator in the healing process. The probability of in vitro-in vivo correlation (IVIVC) in probiotic activity leads to lower costs in finding new therapeutic options. Simulated wound fluid (SWF) was used to evaluate the antibacterial function of Lactiplantibacillus plantarum in wounds. The growth parameters in SWF were evaluated using a logistic model to predict growth behavior in the wound area. In addition, probiotic antimicrobial activity and secretion of antibacterial substances in SWF were also studied. Data were used to select the initial dose and apply frequency for in vivo study. The wound models were infected by two main pathogens (Pseudomonas aeruginosa or Staphylococcus aureus). In vitro results showed less lag time associated with considerable acid production in SWF. In the following, secretion of antimicrobial substances and co-aggregation with pathogens became more important. The susceptibility of pathogens to these factors was different, and culture medium affected the yield of each factor involved in eliminating pathogens. Histological analysis and macroscopic examination of wounds revealed probiotics as effective as positive control or more. There were some differences in the antibacterial functions of probiotics in simulated and real wound environments. The in vitro effect of probiotics on removal of pathogens was not the same as the trend seen in vivo.
将抗菌剂递送到伤口区域并防止出现多种耐药生物体（MDRO）的困难使得管理烧伤感染成为医学上的一项复杂任务。益生菌不仅成为治疗烧伤感染的一种可能方法，而且还是促进愈合过程的一种手段。益生菌活性的体外-体内相关性（IVIVC）的可能性导致寻找新的治疗选择的成本降低。使用模拟伤口液（SWF）来评估植物乳杆菌在伤口中的抗菌功能。使用逻辑模型评估 SWF 中的生长参数，以预测在伤口区域的生长行为。此外，还研究了益生菌的抗菌活性和在 SWF 中分泌抗菌物质。数据用于选择体内研究的初始剂量和应用频率。通过两种主要病原体（铜绿假单胞菌或金黄色葡萄球菌）感染伤口模型。体外结果表明，与 SWF 中相当的产酸相关的迟滞时间较短。随后，抗菌物质的分泌和与病原体的共聚集变得更加重要。病原体对这些因素的敏感性不同，并且培养基会影响每种参与消除病原体的因素的产量。伤口的组织学分析和宏观检查显示，益生菌与阳性对照或更有效。在模拟和真实伤口环境中，益生菌的抗菌功能存在一些差异。益生菌对病原体的体外清除效果与体内观察到的趋势并不相同。
