Institute for Cellular and Intracellular Symbiosis UrB RAS, Orenburg, Russia.
Arch Microbiol. 2022 Mar 29;204(4):226. doi: 10.1007/s00203-022-02852-7.
Corynebacterium amycolatum ICIS 99 was isolated from vaginal smears of healthy women and showed promising results in antimicrobial screenings. Here, we report the draft genome sequence of this strain and analyze its main features to assess its safety and useful properties. The genome is 2,532,503 bp long and contains 2186 CDSs with an average G + C content of 59.0%. Analyses of the ICIS 99 genome revealed the absence of true virulence factors. The genome contains genes involved in the synthesis of secondary metabolites and bacteriocins of the class sactipeptide. In the genome of ICIS 99, we identified a large number of genes responsible for adaptation and survival in the vaginal environment, including acid and oxidative stress resistance genes. The genomic information of ICIS 99 provides a basis for understanding the safety and useful properties of ICIS 99 and for considering it as a potential probiotic strain. The whole genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number JAIUSU000000000.
从健康女性的阴道涂片分离出棒状杆菌属 amycolatum ICIS 99，并在抗菌筛选中显示出有希望的结果。在这里，我们报告了该菌株的草图基因组序列，并分析了其主要特征，以评估其安全性和有用特性。基因组全长 2,532,503 bp，包含 2186 个 CDS，平均 G+C 含量为 59.0%。对 ICIS 99 基因组的分析表明，不存在真正的毒力因子。基因组包含参与合成次级代谢物和 sactipeptide 类细菌素的基因。在 ICIS 99 的基因组中，我们鉴定出了大量与阴道环境中适应和生存相关的基因，包括耐酸和耐氧化应激基因。ICIS 99 的基因组信息为理解 ICIS 99 的安全性和有用特性提供了基础，并为将其视为潜在的益生菌菌株提供了依据。全基因组鸟枪法测序已在 DDBJ/ENA/GenBank 下提交，登录号为 JAIUSU000000000。
