Life Sciences Institute, Zunyi Medical University, Zunyi 563006, PR China.
School of Basic Medical Sciences, Zunyi Medical University, Zunyi 563006, PR China.
Int J Syst Evol Microbiol. 2022 Oct;72(10). doi: 10.1099/ijsem.0.005514.
Two endophytic bacteria, designated strains CQZ9-1 and MQZ9-1, were isolated from semi-mangrove plant collected from Maowei Sea Mangrove Nature Reserve in Guangxi Zhuang Autonomous Region, PR China. The two strains possessed almost identical 16S rRNA gene sequences (99.7 %). The average nucleotide identity (ANI), average amino acid identity (AAI) and the digital DNA-DNA hybridization (dDDH) values between the two strains were 100 %, indicating that they represented the same species. The 16S rRNA gene sequence similarities between strains CQZ9-1, MQZ9-1 and the most closely related type strains, KSK16Y-1, MQZ13P-4 and CBS 5Q-3 were 98.0-98.1, 97.3-97.4 and 97.3-97.4 %, respectively. The results of phylogenetic analyses based on 16S rRNA gene sequences and genome sequences indicated that CQZ9-1 and MQZ9-1 formed a distinct lineage with CBS5Q-3, 40Bstr34, KSK16Y-1, MQZ13P-4, JCM 30119 and 22II-16-19i. The draft genomes of strains CQZ9-1 and MQZ9-1 were 4 162 933 bp and 4 164 266 bp in size, respectively, and their DNA G+C contents were both 63.8 %. Comparative genome analysis of the two strains and the type strains of related species revealed ANI, AAI and dDDH values below the cut-off levels of 95-96, 95.5 and 70 %, respectively. The ubiquinone detected in CQZ9-1 was Q-10. The major cellular fatty acid of strains CQZ9-1 and MQZ9-1 was found to be Cω7. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that CQZ9-1 and MQZ9-1 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CQZ9-1 (= CGMCC 1.18725 = JCM 34331).
两株内生细菌，分别命名为菌株 CQZ9-1 和 MQZ9-1，从中国广西壮族自治区茅尾海红树林自然保护区的半红树林植物中分离得到。这两株细菌的 16S rRNA 基因序列（99.7%）几乎完全相同。这两株细菌的平均核苷酸同源性（ANI）、平均氨基酸同源性（AAI）和数字 DNA-DNA 杂交值（dDDH）均为 100%，表明它们代表同一物种。菌株 CQZ9-1、MQZ9-1 与最密切相关的模式菌株 KSK16Y-1、MQZ13P-4 和 CBS 5Q-3 的 16S rRNA 基因序列相似度分别为 98.0-98.1%、97.3-97.4%和 97.3-97.4%。基于 16S rRNA 基因序列和基因组序列的系统发育分析结果表明，CQZ9-1 和 MQZ9-1 与 CBS5Q-3、40Bstr34、KSK16Y-1、MQZ13P-4、JCM 30119 和 22II-16-19i 一起形成了一个独特的谱系。菌株 CQZ9-1 和 MQZ9-1 的基因组草图大小分别为 4,162,933 bp 和 4,164,266 bp，其 DNA G+C 含量均为 63.8%。对这两株细菌和相关种的模式菌株进行比较基因组分析，结果显示，ANI、AAI 和 dDDH 值均低于 95-96%、95.5%和 70%的截断值。在 CQZ9-1 中检测到的泛醌为 Q-10。菌株 CQZ9-1 和 MQZ9-1 的主要细胞脂肪酸被鉴定为 Cω7。表型、系统发育和化学分类学综合数据表明，CQZ9-1 和 MQZ9-1 代表了一个新的属种，建议将其命名为 sp. nov.。该模式菌株为 CQZ9-1（=CGMCC 1.18725=JCM 34331）。
