Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China.
Adv Healthc Mater. 2022 Jul;11(13):e2102837. doi: 10.1002/adhm.202102837. Epub 2022 Apr 13.
Artificial construction from tendon to bone remains a formidable challenge in tissue engineering owing to their structural complexity. In this work, bioinspired calcium silicate nanowires and alginate composite hydrogels are utilized as building blocks to construct multiscale hierarchical bioactive scaffolds for versatile tissue engineering from tendon to bone. By integrating 3D printing technology and mechanical stretch post-treatment in a confined condition, the obtained composite hydrogels possess bioinspired reinforcement architectures from nano- to submicron- to microscale with significantly enhanced mechanical properties. The biochemical and topographical cues of the composite hydrogel scaffolds provide much more efficient microenvironment to the rabbit bone mesenchymal stem cells and rabbit tendon stem cells, leading to ordered alignment and improved differentiation. The composite hydrogels markedly promote in vivo tissue regeneration from bone to tendon, especially fibrocartilage transitional tissue. Therefore, such calcium silicate nanowires/alginate composite hydrogels with multiscale hierarchical structures have potential application for tissue regeneration from tendon to bone. This work provides an innovative strategy to construct multiscale hierarchical architecture-based scaffolds for tendon/bone engineering.
由于其结构复杂性，肌腱到骨骼的人工构建仍然是组织工程中的一个艰巨挑战。在这项工作中，仿生硅酸钙纳米线和藻酸盐复合水凝胶被用作构建块，以构建用于从肌腱到骨骼的多功能组织工程的多尺度分级生物活性支架。通过在受限条件下整合 3D 打印技术和机械拉伸后处理，所获得的复合水凝胶具有仿生增强结构，从纳米到亚微米到微米尺度，机械性能显著提高。复合水凝胶支架的生化和形貌线索为兔骨髓间充质干细胞和兔肌腱干细胞提供了更有效的微环境，导致有序排列和改善的分化。复合水凝胶明显促进了从骨到肌腱的体内组织再生，特别是纤维软骨过渡组织。因此，具有多尺度分级结构的这种硅酸钙纳米线/藻酸盐复合水凝胶具有用于肌腱/骨骼再生的应用潜力。这项工作为肌腱/骨骼工程提供了一种构建基于多尺度分级结构的支架的创新策略。
