Koinis-Mitchell Daphne, Marshall Gailen D, Kopel Sheryl J, Belanger Nicole M S, Ayala-Figueroa Jesús, Echevarria Sofia, Millman Richard, Zheng Tao, Weathers Jessica, Gredvig Caroline A, Carskadon Mary A
Department of Pediatrics, Hasbro Children's Hospital, Providence, RI, USA.
Bradley-Hasbro Children's Research Center, Rhode Island Hospital, Providence, RI, USA.
Sleep Adv. 2022 Jan 27;3(1):zpac003. doi: 10.1093/sleepadvances/zpac003. eCollection 2022.
We describe research methods developed to examine effects of sleep disruption on changes in immune balance, lung function, and cognitive performance in a sample of urban, ethnically diverse children with persistent asthma. Two case examples (8- and 10-year-old males) are presented to highlight methods of the current study and illustrate effects of experimentally disrupted sleep on the immune balance profile (Th1/Th2 cytokines), key sleep variables from polysomnography data, and lung function in our sample.
Children follow an individualized structured sleep schedule consistent with their habitual sleep need (≥9.5 hours' time in bed) for six days before a laboratory-based experimental sleep protocol. Children then spend two successive nights in the sleep lab monitored by polysomnography: a baseline night consisting of uninterrupted sleep, and a disruption night, during which they are awoken for 2 minutes between 20-minute intervals of uninterrupted sleep. Evening and morning blood draws bracket baseline and disruption nights for immune biomarker assessment.
A shift towards immune imbalance following the sleep disruption protocol was observed in these illustrative cases.
Data from these case examples provide evidence that the experimental protocol caused disruptions in sleep as observed on polysomnography and had the hypothesized downstream effects on immune balance associated with clinical asthma control. Documenting the effects of sleep disruption on immune function in children with persistent asthma is a crucial step towards understanding associations between sleep, immune balance, and asthma outcomes and provides important information for developing novel interventions for youth with asthma and suboptimal sleep.
Not applicable.
我们描述了所开发的研究方法，以检验睡眠中断对城市中患有持续性哮喘的不同种族儿童样本的免疫平衡、肺功能和认知表现变化的影响。本文给出了两个案例（8岁和10岁男性），以突出当前研究的方法，并说明实验性睡眠中断对我们样本中的免疫平衡概况（Th1/Th2细胞因子）、多导睡眠图数据中的关键睡眠变量以及肺功能的影响。
在基于实验室的实验性睡眠方案实施前，儿童需遵循与其习惯性睡眠需求一致（卧床时间≥9.5小时）的个性化结构化睡眠时间表六天。然后，儿童在睡眠实验室连续度过两个晚上，期间通过多导睡眠图进行监测：一个是由不间断睡眠组成的基线夜，另一个是干扰夜，在此期间，他们在不间断睡眠的20分钟间隔之间被唤醒2分钟。在基线夜和干扰夜前后分别于傍晚和早晨采血，用于免疫生物标志物评估。
在这些示例案例中，观察到睡眠中断方案后出现了免疫失衡的转变。
这些案例的数据提供了证据，表明实验方案导致了多导睡眠图上所观察到的睡眠中断，并对与临床哮喘控制相关的免疫平衡产生了假设的下游影响。记录睡眠中断对患有持续性哮喘儿童免疫功能的影响，是理解睡眠、免疫平衡和哮喘结局之间关联的关键一步，并为开发针对哮喘和睡眠不佳的青少年的新型干预措施提供了重要信息。
不适用。
