The purpose of this research was to establish the optimal recovery duration following a pre-load stimulus on performance measures related to handball players. Seventeen senior male University handball players (mean ± SD: age 23.6 ± 2.3 yrs., height 1.79 ± 0.06 m and body mass 72.5 ± 10.7 kg) performed three experimental sessions. All sessions consisted of a standardised warm-up followed by a pre-load stimulus (HSR) back squats followed by a passive rest for either 4-min (PAP4), 8-min (PAP8), or 12-min (PAP12). Following the completion of the passive recovery, players then performed a countermovement jump (CMJ), a 20-m linear sprint and a modified agility t-test. The significance level was set at P < 0.05. There was a significant main effect of passive rest duration after the pre-load stimulus. The PAP12 condition improved CMJ scores (2.3-2.6%; effect size = small), 20-m linear sprint times (3.3-3.7%; effect size = small to moderate) and agility times (1.6-1.9%; effect size = trivial) compared to PAP4 and PAP8 conditions (P < 0.0005). Values of heart rate and rating of perceived exertion were also significantly lower during the PAP12 condition compared to the PAP4 and PAP8 conditions (P < 0.0005). A positive Pearson correlation was established between agility and CMJ for all conditions (P < 0.001). The findings provide novel data observing that a pre-load stimulus, followed by 12-min of recovery, results in greater maximal jump, sprint and agility measures when compared with a 4-min or 8-min recovery in male handball players.