Paterna Angela, Rao Estella, Adamo Giorgia, Raccosta Samuele, Picciotto Sabrina, Romancino Daniele, Noto Rosina, Touzet Nicolas, Bongiovanni Antonella, Manno Mauro
Cell-Tech Hub, Institute of Biophysics, National Research Council of Italy, Palermo, Italy.
Cell-Tech Hub, Institute for Research and Biomedical Innovation, National Research Council of Italy, Palermo, Italy.
Front Bioeng Biotechnol. 2022 Mar 14;10:836747. doi: 10.3389/fbioe.2022.836747. eCollection 2022.
Extracellular vesicles (EVs) play a crucial role as potent signal transducers among cells, with the potential to operate cross-species and cross-kingdom communication. Nanoalgosomes are a subtype of EVs recently identified and isolated from microalgae. Microalgae represent a natural bioresource with the capacity to produce several secondary metabolites with a broad range of biological activities and commercial applications. The present study highlights the upstream and downstream processes required for the scalable production of nanoalgosomes from cultures of the marine microalgae . Different technical parameters, protocols, and conditions were assessed to improve EVs isolation by tangential flow filtration (TFF), aiming to enhance sample purity and yield. The optimization of the overall bioprocess was enhanced by quality control checks operated through robust biophysical and biochemical characterizations. Further, we showed the possibility of recycling by TFF microalgae cells post-EVs isolation for multiple EV production cycles. The present results highlight the potential of nanoalgosome production as a scalable, cost-effective bioprocess suitable for diverse scientific and industrial exploitations.
细胞外囊泡（EVs）作为细胞间强大的信号转导器发挥着关键作用，具有跨物种和跨界通讯的潜力。纳米藻泡是最近从微藻中鉴定和分离出来的一种EVs亚型。微藻是一种天然生物资源，能够产生多种具有广泛生物活性和商业应用的次生代谢产物。本研究强调了从海洋微藻培养物中可扩展生产纳米藻泡所需的上游和下游过程。评估了不同的技术参数、方案和条件，以通过切向流过滤（TFF）改善EVs的分离，旨在提高样品纯度和产量。通过强大的生物物理和生化表征进行质量控制检查，加强了整个生物过程的优化。此外，我们展示了在EVs分离后通过TFF回收微藻细胞以进行多个EV生产周期的可能性。目前的结果突出了纳米藻泡生产作为一种适用于多种科学和工业开发的可扩展、具有成本效益的生物过程的潜力。
