Grafstrom R H, Hoess R H
Nucleic Acids Res. 1987 Apr 10;15(7):3073-84. doi: 10.1093/nar/15.7.3073.
The complete nucleotide sequence of mutH gene from E. coli has been determined. Based on the deduced amino acid sequence, the MutH protein has a molecular weight of 25.4 kdaltons in agreement with the previous estimates based on SDS-polyacrylamide gel electrophoresis of the purified protein. Deletion analysis of the DNA sequences upstream of mutH has identified the promoter region for this gene. Two independently isolated temperature sensitive alleles of the mutH gene have also been sequenced. One mutation results in an amino acid change at position 27 (thr to leu) while the other occurs at position 156 (asp to asn).
已确定大肠杆菌mutH基因的完整核苷酸序列。根据推导的氨基酸序列，MutH蛋白的分子量为25.4千道尔顿，这与之前基于纯化蛋白的SDS-聚丙烯酰胺凝胶电泳所做的估计一致。对mutH上游DNA序列的缺失分析确定了该基因的启动子区域。还对mutH基因的两个独立分离的温度敏感等位基因进行了测序。一个突变导致第27位氨基酸发生变化（苏氨酸变为亮氨酸），而另一个突变发生在第156位（天冬氨酸变为天冬酰胺）。
