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大肠杆菌K-12中甲基化的动力学

Kinetics of methylation in Escherichia coli K-12.

作者信息

Lyons S M, Schendel P F

出版信息

J Bacteriol. 1984 Jul;159(1):421-3. doi: 10.1128/jb.159.1.421-423.1984.

Abstract

Newly synthesized DNA is undermethylated in E. coli K-12. The amount of N6-methyl deoxyadenylic acid in labeled DNA varied from 0.3 mol% of total adenine for a 2-min pulse to 1.7 mol% for DNA that was labeled for more than two generations.

摘要

新合成的DNA在大肠杆菌K-12中处于低甲基化状态。标记DNA中N6-甲基脱氧腺苷酸的含量,对于2分钟脉冲标记的DNA,占腺嘌呤总量的0.3摩尔%;对于标记超过两代的DNA,则为1.7摩尔%。

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