Fan Jianzhong, Cai Heng, Fang Youhong, He Jintao, Zhang Linghong, Xu Qingye, Yang Yunxing, Leptihn Sebastian, Yu Yunsong, Zhao Dongdong, Hua Xiaoting
Department of Laboratory Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Front Microbiol. 2022 Mar 17;13:852434. doi: 10.3389/fmicb.2022.852434. eCollection 2022.
is a zoonotic food-borne pathogen threatening public health around the world. As is the case with many other pathogens, the spread of mobilized colistin resistance () alleles is of grave concern. In this study, totally 689 clinical isolates were collected from a local hospital in Hangzhou, Zhejiang Province, China between 2009 and 2018. Resistance genes were screen by PCR. Two positive strains S15 and S639 were identified which belong to serotype Typhimurium and Thompson, respectively. We observed that both genes were located on conjugative IncHI2 plasmids which encoded numerous resistance genes, likely facilitating the dissemination of by co-resistance mechanisms. The cassettes encoded on the two plasmids were not identical: downstream of the genes, we found IS on one plasmid (pS15), while the other had a -IS (pS639). Despite the presence of cassettes, the strains were not rendered colistin resistant. Yet, it is of epidemiological importance to implement surveillance to be able to observe and possibly control the spread of due to its potential to mediate resistance to the last-resort antibiotic colistin.
是一种人畜共患的食源性病原体，威胁着全球公共卫生。与许多其他病原体一样，可移动的黏菌素耐药性（）等位基因的传播令人严重担忧。在本研究中，2009年至2018年间，从中国浙江省杭州市一家当地医院共收集了689株临床分离株。通过PCR筛选耐药基因。鉴定出两株阳性菌株S15和S639，分别属于鼠伤寒血清型和汤普森血清型。我们观察到，两个基因均位于接合性IncHI2质粒上，该质粒编码众多耐药基因，可能通过共耐药机制促进的传播。两个质粒上编码的盒式结构并不相同：在基因下游，我们在一个质粒（pS15）上发现了IS，而另一个质粒（pS639）上有一个-IS。尽管存在盒式结构，但这些菌株并未对黏菌素产生耐药性。然而，由于其有可能介导对最后一道防线抗生素黏菌素的耐药性，实施监测以便能够观察并可能控制的传播具有重要的流行病学意义。
