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一种稳健的体外可灌注微血管网络形成方法。

A Robust Method for Perfusable Microvascular Network Formation In Vitro.

机构信息

Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 02215, USA.

出版信息

Small Methods. 2022 Jun;6(6):e2200143. doi: 10.1002/smtd.202200143. Epub 2022 Apr 3.

DOI:10.1002/smtd.202200143
PMID:35373502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9844969/
Abstract

Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.

摘要

基于微孔的微流控装置广泛应用于不同研究领域的微血管网络（MVN）形成。然而，持续生成具有生理形态和尺寸的可灌注 MVN 一直具有挑战性。在此，研究了初始接种参数如何决定 MVN 形成的关键特征，并建立了一种稳健的两步接种策略，以在微流控装置中生成可灌注的生理 MVN。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/bf798a7b2637/nihms-1862800-f0008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/f9312072f7d7/nihms-1862800-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/83db9332639d/nihms-1862800-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/8929040824a4/nihms-1862800-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/bf798a7b2637/nihms-1862800-f0008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/83db9332639d/nihms-1862800-f0006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/bf798a7b2637/nihms-1862800-f0008.jpg

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