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核心技术专利：CN118964589B侵权必究
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一种稳健的体外可灌注微血管网络形成方法。

A Robust Method for Perfusable Microvascular Network Formation In Vitro.

机构信息

Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.

Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, 02215, USA.

出版信息

Small Methods. 2022 Jun;6(6):e2200143. doi: 10.1002/smtd.202200143. Epub 2022 Apr 3.

DOI:10.1002/smtd.202200143
PMID:35373502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9844969/
Abstract

Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.

摘要

基于微孔的微流控装置广泛应用于不同研究领域的微血管网络（MVN）形成。然而，持续生成具有生理形态和尺寸的可灌注 MVN 一直具有挑战性。在此，研究了初始接种参数如何决定 MVN 形成的关键特征，并建立了一种稳健的两步接种策略，以在微流控装置中生成可灌注的生理 MVN。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/bf798a7b2637/nihms-1862800-f0008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/f9312072f7d7/nihms-1862800-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/83db9332639d/nihms-1862800-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/8929040824a4/nihms-1862800-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/bf798a7b2637/nihms-1862800-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/7337fa621e14/nihms-1862800-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/9af8be22fb43/nihms-1862800-f0002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/24073c0a9633/nihms-1862800-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/f9312072f7d7/nihms-1862800-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/83db9332639d/nihms-1862800-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/8929040824a4/nihms-1862800-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a6a/9844969/bf798a7b2637/nihms-1862800-f0008.jpg

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[10]
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本文引用的文献

[1]
Engineered human blood-brain barrier microfluidic model for vascular permeability analyses.

Nat Protoc. 2022-1

[2]
Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices.

Micromachines (Basel). 2021-11-30

[3]
Physiologic flow-conditioning limits vascular dysfunction in engineered human capillaries.

Biomaterials. 2022-1

[4]
Classical and Non-classical Fibrosis Phenotypes Are Revealed by Lung and Cardiac Like Microvascular Tissues On-Chip.

Front Physiol. 2021-10-6

[5]
The vascular niche in next generation microphysiological systems.

Lab Chip. 2021-9-7

[6]
A robust vasculogenic microfluidic model using human immortalized endothelial cells and Thy1 positive fibroblasts.

Biomaterials. 2021-9

[7]
Microfluidic Tumor Vasculature Model to Recapitulate an Endothelial Immune Barrier Expressing FasL.

ACS Biomater Sci Eng. 2021-3-8

[8]
A multi-niche microvascularized human bone marrow (hBM) on-a-chip elucidates key roles of the endosteal niche in hBM physiology.

Biomaterials. 2021-3

[9]
Vascularized organoids on a chip: strategies for engineering organoids with functional vasculature.

Lab Chip. 2021-2-9

[10]
Use of 2-dimensional cell monolayers and 3-dimensional microvascular networks on microfluidic devices shows that iron increases transendothelial adiponectin flux via inducing ROS production.

Biochim Biophys Acta Gen Subj. 2021-2

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