通过工程改造酿酒酵母中的肌动蛋白细胞骨架来提高生物燃料的产量。
Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
出版信息
Nat Commun. 2022 Apr 7;13(1):1886. doi: 10.1038/s41467-022-29560-6.
Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels. However, product toxicity has hindered improvements in biofuel production. Here, we engineer the actin cytoskeleton in S. cerevisiae to increase both the cell growth and production of n-butanol and medium-chain fatty acids. Actin cable tortuosity is regulated using an n-butanol responsive promoter-based autonomous bidirectional signal conditioner in S. cerevisiae. The budding index is increased by 14.0%, resulting in the highest n-butanol titer of 1674.3 mg L. Moreover, actin patch density is fine-tuned using a medium-chain fatty acid responsive promoter-based autonomous bidirectional signal conditioner. The intracellular pH is stabilized at 6.4, yielding the highest medium-chain fatty acids titer of 692.3 mg L in yeast extract peptone dextrose medium. Engineering the actin cytoskeleton in S. cerevisiae can efficiently alleviate biofuels toxicity and enhance biofuels production.
酿酒酵母被广泛用作生产生物燃料的细胞工厂。然而，产物毒性阻碍了生物燃料生产的改进。在这里，我们对酿酒酵母中的肌动蛋白细胞骨架进行了工程改造，以提高丁醇和中链脂肪酸的产量和细胞生长。通过使用基于响应丁醇的自主双向信号调节剂的启动子来调节肌动蛋白电缆扭曲。出芽指数增加了 14.0%，导致丁醇产量最高可达 1674.3mg/L。此外，通过使用基于中链脂肪酸响应启动子的自主双向信号调节剂来微调肌动蛋白斑点密度。细胞内 pH 值稳定在 6.4，在酵母提取物-蛋白胨-葡萄糖培养基中获得最高的中链脂肪酸产量为 692.3mg/L。对酿酒酵母中的肌动蛋白细胞骨架进行工程改造可以有效地缓解生物燃料毒性并提高生物燃料产量。
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