Halstead D C, Hoffert M R, Colasante G G
J Clin Microbiol. 1987 Jan;25(1):42-4. doi: 10.1128/jcm.25.1.42-44.1987.
A total of 170 fresh clinical urine isolates were tested with a premarket configuration of the RapID SS/u system (Innovative Diagnostic Systems, Inc., Atlanta, Ga.), a qualitative micromethod for the identification of selected organisms commonly isolated from urine specimens. Results were compared with those obtained with conventional methods of identifying gram-positive isolates and with the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.), utilizing Gram-Negative Identification cards for the identification of gram-negative rods. Organisms representing 12 taxa were included in the study. Of the 170 isolates, 163 (95.9%) were correctly identified. A total of 144 strains (84.7%) were correctly identified without additional testing, whereas 19 isolates (11.2%) required further testing. Seven isolates (4.1%) were incorrectly identified. The SS/u system required minimal hands-on time inoculate and interpret reactions. Discrepancies most often occurred with regard to misinterpretation of Escherichia coli and Enterobacter sp. as Citrobacter sp. The IDS RapID SS/u system may indeed prove valuable for the rapid manual identification of urine isolates.
总共170株新鲜临床尿液分离株使用RapID SS/u系统（创新诊断系统公司，佐治亚州亚特兰大）的上市前配置进行检测，这是一种用于鉴定常见于尿液标本中特定微生物的定性微量方法。将结果与使用传统方法鉴定革兰氏阳性分离株以及与使用革兰氏阴性鉴定卡鉴定革兰氏阴性杆菌的自动微生物系统（Vitek系统公司，密苏里州黑兹尔伍德）所获得的结果进行比较。该研究纳入了代表12个分类单元的微生物。在170株分离株中，163株（95.9%）被正确鉴定。总共144株菌株（84.7%）无需进一步检测即可正确鉴定，而19株分离株（11.2%）需要进一步检测。7株分离株（4.1%）被错误鉴定。SS/u系统接种和解读反应所需的实际操作时间最少。差异最常出现在将大肠杆菌和肠杆菌属误鉴定为柠檬酸杆菌属方面。IDS RapID SS/u系统确实可能被证明对尿液分离株的快速手工鉴定有价值。
