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大麻副产物的三酰基甘油和其他脂质分析。

Triacylglycerols and Other Lipids Profiling of Hemp By-Products.

机构信息

Aquatic and Crop Resource Development Research Centre, National Research Council Canada, 1411 Oxford Street, Halifax, NS B3H 3Z1, Canada.

出版信息

Molecules. 2022 Apr 5;27(7):2339. doi: 10.3390/molecules27072339.

DOI:10.3390/molecules27072339
PMID:35408737
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9000728/
Abstract

Hemp seed by-products, namely hemp cake (hemp meal) and hemp hulls were studied for their lipid content and composition. Total lipid content of hemp cake and hemp hulls was 13.1% and 17.5%, respectively. Oil extraction yields using hexane, on the other hand, were much lower in hemp cake (7.4%) and hemp hulls (12.1%). Oil derived from both hemp seeds and by-products were primarily composed of neutral lipids (>97.1%), mainly triacylglycerols (TAGs), determined by SPE and confirmed by NMR study. Linoleic acid was the major fatty acid present in oils derived from hemp by-products, covering almost 55%, followed by α-linolenic acid, covering around 18% of the total fatty acids. For the first time, 47 intact TAGs were identified in the hemp oils using UPLC-HRMS. Among them, TAGs with fatty acid acyl chain 18:3/18:2/18:2 and 18:3/18:2/18:1 were the major ones, followed by TAGs with fatty acid acyl chain of 18:3/18:3/18:2, 18:2/18:2/16:0, 18:2/18:2/18:1, 18:3/18:2.18:0, 18:2/18:2/18:0, 18:2/18:1/18:1 and 18:3/18:2:16:0. Besides TAGs, low levels of terpenes, carotenoids and cannabidiolic acid were also detected in the oils. Moreover, the oils extracted from hemp by-products possessed a dose-dependent DPPH radical scavenging property and their potencies were in a similar range compared to other vegetable oils.

摘要

麻籽副产物，即麻饼（麻粕）和麻壳，其脂类含量和组成进行了研究。麻饼和麻壳的总脂含量分别为 13.1%和 17.5%。另一方面，使用正己烷提取油的得率在麻饼（7.4%）和麻壳（12.1%）中要低得多。从麻籽和副产物中提取的油主要由中性脂类（>97.1%）组成，主要为三酰基甘油（TAG），通过 SPE 确定，并通过 NMR 研究得到证实。亚油酸是从麻副产物中提取的油中存在的主要脂肪酸，占近 55%，其次是α-亚麻酸，占总脂肪酸的 18%左右。首次使用 UPLC-HRMS 在麻籽油中鉴定出 47 种完整的 TAG。其中，脂肪酸酰基链为 18:3/18:2/18:2 和 18:3/18:2/18:1 的 TAG 为主要成分，其次是脂肪酸酰基链为 18:3/18:3/18:2、18:2/18:2/16:0、18:2/18:2/18:1、18:3/18:2.18:0、18:2/18:2/18:0、18:2/18:1/18:1 和 18:3/18:2:16:0 的 TAG。除了 TAG 外，油中还检测到低水平的萜烯、类胡萝卜素和大麻二酚酸。此外，从麻籽副产物中提取的油具有剂量依赖性的 DPPH 自由基清除活性，其效力与其他植物油相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/d7641893c1ec/molecules-27-02339-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/5379dae626e8/molecules-27-02339-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/721debbf0abb/molecules-27-02339-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/bc396b1cbcd2/molecules-27-02339-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/5a0b76e67bd6/molecules-27-02339-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/d7641893c1ec/molecules-27-02339-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/5379dae626e8/molecules-27-02339-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/721debbf0abb/molecules-27-02339-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/bc396b1cbcd2/molecules-27-02339-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/5a0b76e67bd6/molecules-27-02339-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63dd/9000728/d7641893c1ec/molecules-27-02339-g005.jpg

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