School of Biomedicine, Department of Pharmacy and Pharmacology, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, Russia.
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, 190000 Sankt-Peterburg, Russia.
Molecules. 2020 Sep 7;25(18):4088. doi: 10.3390/molecules25184088.
Extraction process of Semper, 1868, which are subspecies of (Gunnerus, 1767), were studied. It was shown that supercritical carbon dioxide extraction of holothuria was more effective than conventional solvent extraction. Step-by-step extraction with carbon dioxide followed by supercritical extraction with the addition of a co-solvent of ethanol can almost double the yields of extracts of triterpene glycosides, styrenes and carotenoids. Moreover, the fraction of triterpene glycosides practically does not contain colored impurities, in contrast to traditional ethanol extraction. The obtained extracts by HPLC in combination with tandem mass spectrometry (HPLC-MS/MS) identified 15 triterpene glycosides, 18 styrene compounds and 14 carotenoids. Supercritical extraction made it possible to obtain extracts with yields superior to conventional hexane and alcohol extracts. Moreover, such an approach with the use of supercritical fluid extraction (SFE) and subsequent profiling of metabolites can help with the study of holothuria species that are not as well studied.
对 （Gunnerus，1767）的亚种 （Semper，1868）进行了超临界二氧化碳萃取研究。结果表明，与传统溶剂萃取相比，海参的超临界二氧化碳萃取更有效。先用二氧化碳分步萃取，再加入乙醇共溶剂进行超临界萃取，可使三萜糖苷、苯乙烯和类胡萝卜素提取物的收率几乎翻一番。此外，与传统的乙醇提取相比，三萜糖苷的馏分实际上不含有色杂质。通过高效液相色谱与串联质谱（HPLC-MS/MS）相结合，从获得的提取物中鉴定出 15 种三萜糖苷、18 种苯乙烯化合物和 14 种类胡萝卜素。超临界萃取可获得产率优于传统正己烷和醇提物的提取物。此外，这种使用超临界流体萃取（SFE）和随后对代谢物进行分析的方法有助于研究研究较少的海参物种。
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