State Key Laboratory of Transducer Technology, Aerospace Information Research Institute (AIR), Chinese Academy of Sciences, Beijing 100190, China.
School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
Biosensors (Basel). 2022 Apr 15;12(4):247. doi: 10.3390/bios12040247.
This paper proposes a compact bioelectronics sensing platform, including a multi-channel electrode, intracranial electroencephalogram (iEEG) recorder, adjustable galvanometer, and shunt-current conduction circuit pathway. The developed implantable electrode made of polyurethane-insulated stainless-steel materials is capable of recording iEEG signals and shunt-current conduction. The electrochemical impedance of the conduction, ground/reference, and working electrode were characterized in phosphate buffer saline solution, revealing in vitro results of 517.2 Ω@1 kHz (length of 0.1 mm, diameter of 0.8 mm), 1.374 kΩ@1 kHz (length of 0.3 mm, diameter of 0.1 mm), and 3.188 kΩ@1 kHz (length of 0.1 mm, diameter of 0.1 mm), respectively. On-bench measurement of the system revealed that the input noise of the system is less than 2 μVrms, the signal frequency bandwidth range is 1 Hz10 kHz, and the shunt-current detection range is 0.13000 μA with an accuracy of above 99.985%. The electrode was implanted in the CA1 region of the right hippocampus of rats for the in vivo experiments. Kainic acid (KA)-induced seizures were detected through iEEG monitoring, and the induced shunt-current was successfully measured and conducted out of the brain through the designed circuit-body path, which verifies the potential of current conduction for the treatment of epilepsy.
本文提出了一种紧凑的生物电子传感平台，包括多通道电极、颅内脑电图（iEEG）记录器、可调电流计和分流电流传导电路路径。所开发的由聚氨酯绝缘不锈钢材料制成的植入式电极能够记录 iEEG 信号和分流电流传导。在磷酸盐缓冲盐溶液中对传导、接地/参考和工作电极的电化学阻抗进行了表征，在体外结果分别为 517.2 Ω@1 kHz（长度 0.1 mm，直径 0.8 mm）、1.374 kΩ@1 kHz（长度 0.3 mm，直径 0.1 mm）和 3.188 kΩ@1 kHz（长度 0.1 mm，直径 0.1 mm）。系统的台架测量结果表明，系统的输入噪声小于 2 μVrms，信号频率带宽范围为 1 Hz10 kHz，分流电流检测范围为 0.13000 μA，精度高于 99.985%。将电极植入大鼠右侧海马 CA1 区进行体内实验。通过 iEEG 监测检测到海人酸（KA）诱导的癫痫发作，并通过设计的电路-身体路径成功测量并引导出大脑外的诱导分流电流，这验证了电流传导治疗癫痫的潜力。
