Klein Widadh, Ismail Enas, Maboza Ernest, Hussein Ahmed A, Adam Razia Z
Prosthodontics Department, Faculty of Dentistry, University of the Western Cape, Parow, Cape Town 7505, South Africa.
Physics Department, Faculty of Science (Girl's Branch), Al Azhar University, Cairo 11884, Egypt.
J Funct Biomater. 2023 Jul 20;14(7):379. doi: 10.3390/jfb14070379.
Fungal infections caused by () are one of the most prevalent types of oral disorders in the elderly. It has been reported that drug resistance to fungal pathogens poses a severe risk to global healthcare systems and public health. Therefore, the goal of this work is to investigate the cytotoxic and antifungal properties of silver nanoparticles (AgNPs) produced using three different natural extracts: , , and . According to the UV-Vis results, the synthesized AgNPs via , , and show surface plasmonic resonance (SPR) peaks at 430, 440, and 428 nm, respectively. HR-TEM revealed different shapes for the nanoparticles within the size ranges of 16-20, 31-60, and 57-72 nm for , , and , respectively. Using a human oral fibroblast cell line, the cytotoxicity of both AgNPs and plant extracts was tested at concentrations of 0.007, 0.012, 0.025, and 0.062 mg/mL (buccal mucosa fibroblasts). The antifungal activity showed growth inhibition zones of approximately 18 mm, 18.67 mm, and 18.33 mm for the AgNPs conjugated with , , and respectively. For the studied samples, the minimum inhibitory concentration (MIC) was less than 0.015 mg/mL. The AgNPs exhibited antifungal activity that was concentration- and size-dependent. The results of this study offer new insights into the cytotoxicity and antifungal activity of the green-synthesized AgNPs.
由（）引起的真菌感染是老年人中最普遍的口腔疾病类型之一。据报道，真菌病原体的耐药性对全球医疗系统和公共卫生构成了严重风险。因此，本研究的目的是研究使用三种不同天然提取物制备的银纳米颗粒（AgNPs）的细胞毒性和抗真菌特性：，，和。根据紫外可见光谱结果，通过，，和合成的AgNPs分别在430、440和428nm处显示表面等离子体共振（SPR）峰。高分辨率透射电子显微镜（HR-TEM）显示，对于，，和，纳米颗粒的形状不同，尺寸范围分别为16-20nm、31-60nm和57-72nm。使用人类口腔成纤维细胞系，在0.007、0.012、0.025和0.062mg/mL（颊黏膜成纤维细胞）浓度下测试了AgNPs和植物提取物的细胞毒性。抗真菌活性显示，与，和缀合的AgNPs的生长抑制区分别约为18mm、18.67mm和18.33mm。对于所研究的样品，最低抑菌浓度（MIC）小于0.015mg/mL。AgNPs表现出浓度和尺寸依赖性的抗真菌活性。本研究结果为绿色合成AgNPs的细胞毒性和抗真菌活性提供了新的见解。
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