Rong Yanchao, Yang Hao, Xu Hailin, Li Shuting, Wang Peng, Wang Zhiyong, Zhang Yi, Zhu Wenkai, Tang Bing, Zhu Jiayuan, Hu Zhicheng
Department of Burn Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Department of Plastic Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Front Surg. 2022 Apr 5;9:878965. doi: 10.3389/fsurg.2022.878965. eCollection 2022.
Diabetic foot ulcer (DFU) is a complex and devastating complication of diabetes mellitus that are usually stagnant in the inflammatory phase. However, oral wound healing, which is characterized by a rapid and scarless healing process, is regarded an ideal model of wound healing. Thus, we performed a comprehensive bioinformatics analysis of the previously published data regarding oral ulcers and DFUs and found that compared to oral wound healing, the activated pathways of DFUs were enriched in cellular metabolism-related pathways but lacked the activation of inflammatory and immune-related pathways. We also found that CXCL11, DDX60, IFI44, and IFI44L were remarkable nodes since they had the most connections with other members of the module. Meanwhile, CXCL10, IRF7, and DDX58 together formed a closed-loop relationship and occupied central positions in the entire network. The real-time polymerase chain reaction and western blot was applied to validate the gene expression of the hub immune-related genes in the DFU tissues, it was found that CXCL11, IFI44, IFI44L, CXCL10 and IRF7 have a significant difference compared with normal wound tissues. Our research reveals some novel potential immune-related biomarkers and provides new insights into the molecular basis of this debilitating disease.
糖尿病足溃疡（DFU）是糖尿病一种复杂且具有破坏性的并发症，通常停滞在炎症阶段。然而，以快速且无瘢痕愈合过程为特征的口腔伤口愈合被视为伤口愈合的理想模型。因此，我们对先前发表的有关口腔溃疡和糖尿病足溃疡的数据进行了全面的生物信息学分析，发现与口腔伤口愈合相比，糖尿病足溃疡激活的通路在细胞代谢相关通路中富集，但缺乏炎症和免疫相关通路的激活。我们还发现，CXCL11、DDX60、IFI44和IFI44L是显著的节点，因为它们与模块中的其他成员连接最多。同时，CXCL10、IRF7和DDX58共同形成了一种闭环关系，并在整个网络中占据中心位置。应用实时聚合酶链反应和蛋白质印迹法验证糖尿病足溃疡组织中枢纽免疫相关基因的表达，发现与正常伤口组织相比，CXCL11、IFI44、IFI44L、CXCL10和IRF7有显著差异。我们的研究揭示了一些新的潜在免疫相关生物标志物，并为这种使人衰弱的疾病的分子基础提供了新的见解。
