Gutmann L, Vincent S, Billot-Klein D, Acar J F, Mrèna E, Williamson R
Antimicrob Agents Chemother. 1986 Dec;30(6):906-12. doi: 10.1128/AAC.30.6.906.
Compared with cefotaxime, ceftazidime, moxalactam, and aztreonam, ceftriaxone produced the best lytic and bactericidal effects when each was added at about 10 times the MIC to Escherichia coli W7. When each of these antibiotics was added at its MIC, only bacteriostasis occurred, but the simultaneous addition of amdinocillin (mecillinam) was synergistic in causing rapid lysis and bactericidal effects. Induction of lysis of two E. coli mutants containing either a thermosensitive penicillin-binding protein (PBP) 2 or 3 by relatively PBP 3-specific (aztreonam) and PBP 2-specific (amdinocillin) antibiotics indicated that inhibition of only PBPs 2 and 3 can cause lysis. Examination of the interactions of cefotaxime, aztreonam, and cefsulodin, with or without amdinocillin, with their targets suggested that other combinations of PBPs could be involved in the onset of lysis. However, inhibition of both PBPs 2 and 3 may explain the better lysis-inducing activity of ceftriaxone (which binds well to both of these PBPs), as well as the synergistic effect of amdinocillin when added together with low concentrations of other beta-lactam antibiotics that interact with PBP 3.
与头孢噻肟、头孢他啶、拉氧头孢和氨曲南相比，当以约最低抑菌浓度（MIC）的10倍添加到大肠杆菌W7中时，头孢曲松产生了最佳的溶菌和杀菌效果。当以其MIC添加这些抗生素中的每一种时，仅发生抑菌作用，但同时添加氨茚西林（美西林）在导致快速溶菌和杀菌作用方面具有协同作用。用相对PBP 3特异性（氨曲南）和PBP 2特异性（氨茚西林）抗生素诱导含有热敏青霉素结合蛋白（PBP）2或3的两种大肠杆菌突变体的溶菌表明，仅抑制PBP 2和3可导致溶菌。对头孢噻肟、氨曲南和头孢磺啶与或不与氨茚西林与其靶标的相互作用进行研究表明，其他PBP组合可能与溶菌的发生有关。然而，抑制PBP 2和3两者可能解释了头孢曲松（与这两种PBP都结合良好）更好的溶菌诱导活性，以及氨茚西林与低浓度与PBP 3相互作用的其他β-内酰胺抗生素一起添加时的协同作用。
