头孢菌素敏感型大肠杆菌对头孢噻吩的微生物降解作用
Microbial degradation of cephalothin by cephalothin-susceptible Escherichia coli.
作者信息
Nishiura T, Kawada Y, Shiomi Y, O'Hara K, Kono M
出版信息
Antimicrob Agents Chemother. 1978 Jun;13(6):1036-9. doi: 10.1128/AAC.13.6.1036.
Abstract
Cephalothin (CET)-susceptible Escherichia coli, which can degrade CET after prolonged incubation in broth containing a concentration of the drug greater than the minimum inhibitory concentration, was found in a clinical specimen. The substrate specificity of the partially purified enzyme to cephalosporin analogs strongly indicated the occurrence of CET-specific degradation. Nuclear magnetic resonance analysis of the degradation reaction demonstrated the appearance of two new signals attributed to deacetyl CET. This suggests the possibility of the presence of acylesterase.
摘要
在一份临床标本中发现了对头孢噻吩（CET）敏感的大肠杆菌，该菌在含有高于最低抑菌浓度的该药物的肉汤中长时间培养后能够降解CET。部分纯化的酶对头孢菌素类似物的底物特异性强烈表明存在CET特异性降解。对降解反应的核磁共振分析表明出现了两个归属于去乙酰头孢噻吩的新信号。这表明存在酰基酯酶的可能性。
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