Saccon Tatiana D, Dhahbi Joseph M, Schneider Augusto, Nunez Lopez Yury O, Qasem Ahmad, Cavalcante Marcelo B, Sing Lauren K, Naser Saleh A, Masternak Michal M
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas 96010-610, Brazil.
Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, USA.
Biology (Basel). 2022 Mar 25;11(4):508. doi: 10.3390/biology11040508.
Crohn's disease (CD) and rheumatoid arthritis (RA) are immune mediated inflammatory diseases. Several studies indicate a role for microRNAs (miRNAs) in the pathogenesis of a variety of autoimmune diseases, including CD and RA. Our study's goal was to investigate circulating miRNAs in CD and RA patients to identify potential new biomarkers for early detection and personalized therapeutic approaches for autoimmune diseases. For this study, subjects with CD ( = 7), RA ( = 8) and healthy controls ( = 7) were recruited, and plasma was collected for miRNA sequencing. Comparison of the expression patterns of miRNAs between CD and healthy patients identified 99 differentially expressed miRNAs. Out of these miRNAs, 4 were down regulated, while 95 were up regulated. Comparison of miRNAs between RA and healthy patients identified 57 differentially expressed miRNAs. Out of those, 12 were down regulated, while 45 were up regulated. For all the miRNAs down regulated in CD and RA patients, 420 GO terms for biological processes were similarly regulated between both groups. Therefore, the identification of new plasma miRNAs allows the emergence of new biomarkers that can assist in the diagnosis and treatment of CD and RA.
克罗恩病（CD）和类风湿性关节炎（RA）是免疫介导的炎症性疾病。多项研究表明，微小RNA（miRNA）在包括CD和RA在内的多种自身免疫性疾病的发病机制中发挥作用。我们研究的目的是调查CD和RA患者循环中的miRNA，以确定用于自身免疫性疾病早期检测的潜在新生物标志物和个性化治疗方法。在本研究中，招募了患有CD（n = 7）、RA（n = 8）的受试者以及健康对照（n = 7），并采集血浆用于miRNA测序。比较CD患者与健康患者之间miRNA的表达模式，鉴定出99个差异表达的miRNA。在这些miRNA中，4个下调，95个上调。比较RA患者与健康患者之间的miRNA，鉴定出57个差异表达的miRNA。其中，12个下调，45个上调。对于在CD和RA患者中均下调的所有miRNA，两组之间420个生物过程的基因本体（GO）术语受到类似调控。因此，新血浆miRNA的鉴定有助于发现可辅助CD和RA诊断及治疗的新生物标志物。
