Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Department of Proctology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310021, China.
Nutrients. 2022 Apr 9;14(8):1568. doi: 10.3390/nu14081568.
In this study, we evaluated the effects of different feeding methods on the characteristics of intestinal flora and gas production in infants and toddlers by using an in vitro simulated intestinal microecology fermentation and organoid model. We found that the feeding method influences intestinal gas and fecal ammonia production in infants and toddlers. Supplementation with milk powder for infants in the late lactation period could promote the proliferation of beneficial bacteria, including . Intestinal flora gas production in a culture medium supplemented with fucosyllactose (2'-FL) was significantly lower than that in media containing other carbon sources. In conclusion, 2'-FL may reduce gas production in infant and toddler guts through two mechanisms: first, it cannot be used by harmful intestinal bacteria to produce gas; second, it can inhibit intestinal mucosa colonization by harmful bacteria by regulating the expression of intestinal epithelial pathogenic genes/signaling pathways, thus reducing the proliferation of gas-producing harmful bacteria in the gut.
本研究通过体外模拟肠道微生态发酵和类器官模型，评估了不同喂养方式对婴幼儿肠道菌群特征和气体生成的影响。我们发现喂养方式影响婴幼儿肠道气体和粪便氨的产生。在婴儿后期的哺乳期中添加奶粉可以促进有益细菌的增殖，包括. 在添加岩藻糖基乳糖（2'-FL）的培养基中培养的肠道菌群的气体生成量明显低于含有其他碳源的培养基。总之，2'-FL 可能通过两种机制来减少婴幼儿肠道的气体生成：首先，它不能被有害肠道细菌利用来产生气体；其次，它可以通过调节肠道上皮致病性基因/信号通路的表达来抑制有害细菌对肠道黏膜的定植，从而减少肠道中产气有害细菌的增殖。
