College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Livestock Product Processing Engineering and Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China.
Nutrients. 2022 Apr 13;14(8):1622. doi: 10.3390/nu14081622.
The effect of diet on skin aging has become an interesting research topic. Previous studies have mostly focused on the beneficial effects of collagen peptides derived from marine organisms on the aging skin when administered orally, while the beneficial effects of collagen peptides derived from poultry on aging skin have been rarely reported. In this study, collagen peptides were prepared from chicken bone by enzymatic hydrolysis, and the effect and mechanism of action of orally administered collagen peptides on alleviating skin aging induced by UV combined with D-galactose were investigated. The results showed that the chicken bone collagen had typical characteristics of collagen, and the chicken bone collagen peptides (CPs) were mainly small molecular peptides with a molecular weight of <3000 Da. In vivo experiments showed that CPs had a significant relieving effect on aging skin, indicated by the changes in the compostion and structure of the aging skin, improvement of skin antioxidant level, and inhibition of inflammation; the relieving effect was positively correlated with the dose of CPs. Further investigation showed that CPs first reduce the level of skin oxidation, inhibit the expression of the key transcription factor AP-1 (c-Jun and c-Fos), then activate the TGF-β/Smad signaling pathway to promote collagen synthesis, inhibit the expression of MMP-1/3 to inhibit collagen degradation, and inhibit skin inflammation to alleviate skin aging in mice. Moreover, the skin transcriptome found that lysosomes activated after oral administration of CPs may be an important pathway for CPs in anti-skin aging, and is worthy of further research. These results suggested that CPs might be used as a functional anti-aging nutritional component.
饮食对皮肤衰老的影响已成为一个有趣的研究课题。先前的研究大多集中在口服海洋生物来源的胶原蛋白肽对衰老皮肤的有益作用上，而很少有报道关于家禽来源的胶原蛋白肽对衰老皮肤的有益作用。本研究采用酶解技术从鸡骨中制备胶原蛋白肽，研究了口服胶原蛋白肽缓解 UV 联合 D-半乳糖诱导皮肤衰老的作用及机制。结果表明，鸡骨胶原蛋白具有典型的胶原蛋白特征，鸡骨胶原蛋白肽（CPs）主要为分子量<3000 Da 的小分子肽。体内实验表明，CPs 对衰老皮肤有明显的缓解作用，表现在衰老皮肤的成分和结构变化、皮肤抗氧化水平的提高以及炎症的抑制；缓解作用与 CPs 的剂量呈正相关。进一步研究表明，CPs 首先降低皮肤氧化水平，抑制关键转录因子 AP-1（c-Jun 和 c-Fos）的表达，然后激活 TGF-β/Smad 信号通路促进胶原蛋白合成，抑制 MMP-1/3 的表达抑制胶原蛋白降解，抑制皮肤炎症，从而缓解小鼠皮肤衰老。此外，皮肤转录组学发现 CPs 口服后激活的溶酶体可能是 CPs 抗皮肤衰老的重要途径，值得进一步研究。这些结果表明 CPs 可能作为一种功能性抗衰老营养成分。
