Orbach-Arbouys S, Castes M
Immunology. 1979 Feb;36(2):265-9.
A single intraperitoneal injection of 0.5 mg methotrexate (MTX) has been found to increase the immune reactivity of spleen cells from (C57B1/6 X DBA/2)F1 mice. Five days after injection, spleen cells from MTX-treated mice exhibited greater PHA responsiveness and GvH reactivity, and mice given SRBC at this time developed greater than normal direct PFC responses. This pattern of effects of MTX was particularly evident in mice that had been given high doses of BCG intravenously 14 days before testing, a treatment that generally depressed the measured activities. MTX enhancement of GvH was also observed in mice that had been depleted of short-lived T lymphocytes by adult thymectomy. We suggest that MTX-sensitive cells possible exert, particularly in BCG-treated mice, a suppressive action on the responding cells.
已发现腹腔内单次注射0.5毫克甲氨蝶呤（MTX）可增强（C57B1/6×DBA/2）F1小鼠脾细胞的免疫反应性。注射后五天，经MTX处理的小鼠的脾细胞表现出更强的对PHA的反应性和移植物抗宿主（GvH）反应性，此时给予SRBC的小鼠产生的直接PFC反应高于正常水平。MTX的这种作用模式在测试前14天静脉注射高剂量卡介苗（BCG）的小鼠中尤为明显，这种处理通常会抑制所测活性。在通过成年胸腺切除术清除了短命T淋巴细胞的小鼠中也观察到MTX对GvH的增强作用。我们认为，MTX敏感细胞可能尤其在经BCG处理的小鼠中，对反应细胞发挥抑制作用。
