Vasilenko Tomáš, Kováč Ivan, Slezák Martin, Ďurkáč Ján, Peržel'ová Vlasta, Čoma Matúš, Kaňuchová Miriam, Urban Lukáš, Szabo Pavol, Dvořánková Barbora, Vrzgula Andrej, Zajíček Robert, Smetana Karel, Gál Peter
Department of Surgery, Agel Hospital Košice-Šaca and Pavol Jozef Šafárik University in Košice, Košice, Slovak Republic.
Second Department of Surgery, Louis Pasteur University Hospital and Pavol Jozef Šafárik University in Košice, Košice, Slovak Republic.
In Vivo. 2022 May-Jun;36(3):1236-1244. doi: 10.21873/invivo.12822.
BACKGROUND/AIM: We have previously shown that the water extract of Agrimonia eupatoria L. (AE) is a valuable source of polyphenols with excellent antioxidant properties and has clinical potential for the prevention and/or adjuvant therapy of cardiovascular complications associated with diabetes. Inspired by our previously published data, in the present study we examined whether AE improves skin wound healing in a series of in vitro and in vivo experiments.
In detail, we investigated the ability of the AE extract to induce fibroblast to myofibroblast conversion, extracellular matrix (ECM) deposition, and keratinocyte proliferation/differentiation, in vitro. In parallel, in an animal model, we measured wound tensile strength (TS) and assessed the progression of open wounds using basic histology and immunofluorescence.
The AE extract induced the myofibroblast-like phenotype and enhanced ECM deposition, both in vitro and in vivo. Furthermore, the wound TS of skin incisions and the contraction rates of open excisions were significantly increased in the AE-treated group.
The present data show that AE water extract significantly improves the healing of open and sutured skin wounds. Therefore, our data warrant further testing in animal models that are physiologically and evolutionarily closer to humans.
背景/目的：我们之前已经表明，仙鹤草（AE）的水提取物是一种有价值的多酚来源，具有出色的抗氧化特性，并且在预防和/或辅助治疗与糖尿病相关的心血管并发症方面具有临床潜力。受我们之前发表的数据启发，在本研究中，我们通过一系列体外和体内实验研究了AE是否能改善皮肤伤口愈合。
详细地说，我们在体外研究了AE提取物诱导成纤维细胞向肌成纤维细胞转化、细胞外基质（ECM）沉积以及角质形成细胞增殖/分化的能力。同时，在一个动物模型中，我们测量了伤口抗张强度（TS），并使用基础组织学和免疫荧光评估开放性伤口的进展情况。
AE提取物在体外和体内均诱导了肌成纤维细胞样表型并增强了ECM沉积。此外，AE治疗组皮肤切口的伤口TS和开放性切除伤口的收缩率显著增加。
目前的数据表明，AE水提取物能显著改善开放性和缝合性皮肤伤口的愈合。因此，我们的数据值得在生理和进化上更接近人类的动物模型中进行进一步测试。
