巴西苏木素诱导 A549 癌细胞系发生内在型细胞凋亡与其增加 p53、caspase-9 和 caspase-3 有关。
Brazilin Isolated from Caesalpina Sappan Wood Induces Intrinsic Apoptosis on A549 Cancer Cell Line by Increasing p53, caspase-9, and caspase-3.
机构信息
Doctoral Program of Medical Sciences , Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia.
Department of Histology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia.
出版信息
Asian Pac J Cancer Prev. 2022 Apr 1;23(4):1337-1343. doi: 10.31557/APJCP.2022.23.4.1337.
OBJECTIVE
Lung cancer is the leading cause of death among cancer patients. The majority of lung cancer is the Non-Small Lung Carcinoma (NSLC). This study evaluated the potency of brazilin isolated from Caesalpinia sappan wood to induce apoptosis on non-small lung carcinoma cell line, A549, by examining the expression of p53, caspase-9, and caspase-3.
METHODS
Brazilin was isolated from Caesalpinia sappan wood following a guided assay and it was determined by using Brazilin®SIGMA as standard. The activity of brazilin on the growth of A549 cell line was analysed by MTT assay and the apoptosis was evaluated by flowcytometer following Annexin V (FITC) and PI staining. The expression of p53, caspase-9, and caspase-3 was examined by immunocytochemistry.
RESULT
The IC50 of brazilin on A549 cell line was 43µg/mL. Cell treatment with 20 µg/mL and 40 µg/mL of brazilin significantly increased early apoptosis (p<0.001). Cell treatment with 40 µg/mL of Brazilin significantly increased late apoptosis (p<0.001). Brazilin significantly increased the expression of p53, Caspase-9, and caspase-3 (p<0.001).
CONCLUSION
This study showed evidence of the activity of brazilin to induce intrinsic apoptosis on a NSLC cell line A549.
目的
肺癌是癌症患者死亡的主要原因。大多数肺癌是非小细胞肺癌（NSCLC）。本研究通过检测 p53、caspase-9 和 caspase-3 的表达，评估从苏木中分离得到的巴西苏木素诱导非小细胞肺癌细胞系 A549 凋亡的能力。
方法
按照指导检测方案从苏木中分离巴西苏木素，并使用巴西苏木素®SIGMA 作为标准进行鉴定。MTT 法分析巴西苏木素对 A549 细胞系生长的活性，用 Annexin V（FITC）和 PI 染色后通过流式细胞仪评估细胞凋亡。用免疫细胞化学法检测 p53、caspase-9 和 caspase-3 的表达。
结果
巴西苏木素对 A549 细胞系的 IC50 为 43µg/mL。用 20µg/mL 和 40µg/mL 的巴西苏木素处理细胞可显著增加早期凋亡（p<0.001）。用 40µg/mL 的巴西苏木素处理细胞可显著增加晚期凋亡（p<0.001）。巴西苏木素显著增加了 p53、Caspase-9 和 caspase-3 的表达（p<0.001）。
结论
本研究表明巴西苏木素具有诱导非小细胞肺癌细胞系 A549 发生内在凋亡的活性。
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