Suebsakwong Parichat, Chulrik Wanatsanan, Chunglok Warangkana, Li Jian-Xin, Yao Zhu-Jun, Suksamrarn Apichart
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University Bangkok 10240 Thailand
School of Allied Health Sciences and Research Institute for Health Sciences, Walailak University Nakhon Si Thammarat 80161 Thailand.
RSC Adv. 2020 Mar 11;10(18):10461-10470. doi: 10.1039/d0ra01176b.
Five new triterpenoid saponin glycosides, trichocucumerisides A-E (1-5), together with eleven known compounds (6-16) were isolated from fruit fibers. The structures of the new compounds were elucidated by detailed analysis of NMR and mass spectroscopic data as well as chemical reactions. The anti-inflammatory study against nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells shows that compounds 7 and 9 exhibited stronger NO inhibitory activity, with IC values of 3.0 and 2.7 μM, respectively, with comparison to positive references Celecoxib and aminoguanidine (IC values 75.7 and 75.0 μM, respectively). Compounds 7 and 9 also possessed a greater selectivity index (SI) of approximately 3-4-fold activity than that of the positive references.
从果实纤维中分离出了五种新的三萜皂苷糖苷，即丝瓜三萜苷A - E（1 - 5），以及十一种已知化合物（6 - 16）。通过对核磁共振和质谱数据以及化学反应的详细分析，阐明了新化合物的结构。针对脂多糖（LPS）刺激的RAW264.7细胞中一氧化氮（NO）生成的抗炎研究表明，与阳性对照塞来昔布和氨基胍（IC值分别为75.7和75.0 μM）相比，化合物7和9表现出更强的NO抑制活性，IC值分别为3.0和2.7 μM。化合物7和9还具有比阳性对照约高3 - 4倍活性的更大的选择性指数（SI）。
