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酵母细胞质苹果酸脱氢酶葡萄糖依赖性失活中分解代谢物降解的证据。

Evidence for catabolite degradation in the glucose-dependent inactivation of yeast cytoplasmic malate dehydrogenase.

作者信息

Neeff J, Hägele E, Nauhaus J, Heer U, Mecke D

出版信息

Eur J Biochem. 1978 Jul 3;87(3):489-95. doi: 10.1111/j.1432-1033.1978.tb12399.x.

DOI:10.1111/j.1432-1033.1978.tb12399.x
PMID:354933
Abstract

The cytoplasmic malate dehydrogenase of Saccharomyces cerevisiae was radioactively labeled during its synthesis on a glucose-free derepression medium. After purification a sensitive radio-immunoassay for this enzyme could be developed. The assay showed that after the physiological, glucose-dependent 'catabolite inactivation' of cytoplasmic malate dehydrogenase an inactive enzyme protein is immunologically not detectable. Together with the irreversibility of this reaction in vivo this finding strongly suggest a proteolytic mechanism of enzyme inactivation. For this process the term 'catabolite degradation' is used.

摘要

酿酒酵母的细胞质苹果酸脱氢酶在无糖去阻遏培养基上合成过程中被放射性标记。纯化后，可开发出针对该酶的灵敏放射免疫测定法。该测定法表明，在细胞质苹果酸脱氢酶发生生理性的、依赖葡萄糖的“分解代谢物失活”后，无法通过免疫方法检测到无活性的酶蛋白。结合该反应在体内的不可逆性，这一发现强烈提示存在一种酶失活的蛋白水解机制。对于这一过程，使用术语“分解代谢物降解”。

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1
Evidence for catabolite degradation in the glucose-dependent inactivation of yeast cytoplasmic malate dehydrogenase.酵母细胞质苹果酸脱氢酶葡萄糖依赖性失活中分解代谢物降解的证据。
Eur J Biochem. 1978 Jul 3;87(3):489-95. doi: 10.1111/j.1432-1033.1978.tb12399.x.
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Application of an immunoassay to the study of yeast malate dehydrogenase inactivation.免疫分析法在酵母苹果酸脱氢酶失活研究中的应用。
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Catabolite inactivation of fructose 1,6-bisphosphatase and cytoplasmic malate dehydrogenase in yeast.酵母中果糖1,6 -二磷酸酶和细胞质苹果酸脱氢酶的分解代谢失活
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In vivo and in vitro studies on the glucose dependent inactivation of yeast cytoplasmic malate dehydrogenase.酵母细胞质苹果酸脱氢酶葡萄糖依赖性失活的体内和体外研究。
Arch Microbiol. 1977 Oct 24;115(1):55-60. doi: 10.1007/BF00427845.
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Proteolytic catabolite inactivation in Saccharomyces cerevisiae.酿酒酵母中的蛋白水解分解代谢物失活
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Yeast malate dehydrogenase: enzyme inactivation in catabolite repression.酵母苹果酸脱氢酶：分解代谢物阻遏中的酶失活
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Catabolite inactivation of gluconeogenic enzymes in mutants of yeast deficient in proteinase B.蛋白酶B缺陷型酵母突变体中糖异生酶的分解代谢失活
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Studies on glucose-induced inactivation of gluconeogenetic enzymes in adenylate cyclase and cAMP-dependent protein kinase yeast mutants.关于葡萄糖诱导的腺苷酸环化酶和cAMP依赖性蛋白激酶酵母突变体中糖异生酶失活的研究。
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The malate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, characterisation and studies on their regulation.酿酒酵母的苹果酸脱氢酶同工酶。纯化、特性鉴定及其调控研究。
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Inactivation of yeast enzymes by proteinase A and B and carboxypeptidase Y from yeast.蛋白酶A、B及酵母羧肽酶Y对酵母酶的失活作用
Hoppe Seylers Z Physiol Chem. 1976 May;357(5):735-40. doi: 10.1515/bchm2.1976.357.1.735.

引用本文的文献

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Characterization of the glucose-induced inactivation of maltose permease in Saccharomyces cerevisiae.酿酒酵母中葡萄糖诱导的麦芽糖通透酶失活的表征
J Bacteriol. 1996 Apr;178(8):2245-54. doi: 10.1128/jb.178.8.2245-2254.1996.
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Glucose-induced inactivation of mitochondrial enzymes in the yeast Saccharomyces cerevisiae.葡萄糖诱导酿酒酵母中线粒体酶的失活。
Biochem J. 1981 Aug 15;198(2):281-7. doi: 10.1042/bj1980281.
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Carboxypeptidase S- and carboxypeptidase Y-deficient mutants of Saccharomyces cerevisiae.酿酒酵母羧肽酶S和羧肽酶Y缺陷型突变体。
J Bacteriol. 1981 Aug;147(2):418-26. doi: 10.1128/jb.147.2.418-426.1981.
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Regulation of invertase synthesis by glucose in Saccharomyces cerevisiae.葡萄糖对酿酒酵母中转化酶合成的调控
J Bacteriol. 1982 Oct;152(1):14-8. doi: 10.1128/jb.152.1.14-18.1982.
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Degradation and turnover of peroxisomes in the yeast Hansenula polymorpha induced by selective inactivation of peroxisomal enzymes.多形汉逊酵母中过氧化物酶体酶的选择性失活诱导的过氧化物酶体降解与周转
Arch Microbiol. 1983 Jun;134(3):193-203. doi: 10.1007/BF00407757.
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Regulation of trehalose mobilization in fungi.真菌中海藻糖动员的调控。
Microbiol Rev. 1984 Mar;48(1):42-59. doi: 10.1128/mr.48.1.42-59.1984.
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Calculation of half-lives of proteins in vivo. Heterogeneity in the rate of degradation of yeast proteins.体内蛋白质半衰期的计算。酵母蛋白质降解速率的异质性。
Mol Cell Biochem. 1982 Mar 19;43(2):89-95. doi: 10.1007/BF00423096.
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