Sato Katsuyuki, Nagasawa Takae, Kasumi Takafumi
1 Asai Germanium Research Institute Co., Ltd.
2 Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University.
J Appl Glycosci (1999). 2022 Feb 18;69(1):9-13. doi: 10.5458/jag.jag.JAG-2021_0011. eCollection 2022.
We previously demonstrated that the organogermanium compound 3-(trihydroxygermyl)propanoic acid (THGP) enhances the enzymatic and alkaline isomerization of an aldose to a ketose through -diol complex formation by multiple mechanisms. Its higher affinity for the ketose than the aldose protects the ketose complex from alkaline decomposition. Furthermore, it has been reported that the aldose-ketose alkaline isomerization pathway includes 1,2-enediol. Therefore, we speculated that the complex-forming ability of THGP could also be applied to enediol, a transient intermediate of alkaline isomerization. To test this prediction, we analyzed the initial rates of glucose or lactose isomerization in a region where there was no substantial difference in pH with and without THGP addition. The results showed that THGP enhanced the rate of fructose or lactulose formation per unit time by approximately 2-fold compared to the control. This finding indicated that THGP could form a complex with the transition state of aldose-ketose alkaline isomerization.
我们之前证明，有机锗化合物3-(三羟基锗基)丙酸(THGP)通过多种机制形成二醇配合物，增强了醛糖向酮糖的酶促异构化和碱异构化。它对酮糖的亲和力高于醛糖，可保护酮糖配合物不被碱分解。此外，据报道醛糖-酮糖碱异构化途径包括1,2-烯二醇。因此，我们推测THGP的配合物形成能力也可应用于碱异构化的瞬时中间体烯二醇。为了验证这一预测，我们在添加和不添加THGP的情况下，在pH值没有显著差异的区域分析了葡萄糖或乳糖异构化的初始速率。结果表明，与对照组相比，THGP使单位时间内果糖或乳果糖的形成速率提高了约2倍。这一发现表明，THGP可以与醛糖-酮糖碱异构化的过渡态形成配合物。
