从印度猪粪便样本中检测猪肠道微小核糖核酸病毒
Detection of porcine enteric picornaviruses from faecal samples of Indian pigs.
作者信息
Patel Shailesh Kumar, Agrawal Aditya, Pathak Mamta, Singh Alok, Varshney Rajat, Rana Jigyasa, Saikumar G
机构信息
Division of Pathology, ICAR- Indian Veterinary Research Institute, Bareilly, Izatnagar, U.P 243122 India.
Division of Animal Biochemistry, ICAR- Indian Veterinary Research Institute, Bareilly, Izatnagar, U.P 243122 India.
出版信息
Virusdisease. 2022 Mar;33(1):102-107. doi: 10.1007/s13337-022-00756-0. Epub 2022 Jan 27.
UNLABELLED
Porcine enteric picornaviruses often consequence diarrhoea and nervous complications in pig and pose enormous loss to pig farming. The present study expands the limited Indian data of porcine enteric picornaviruses which is needed for the early implementation of control measures and to check further outbreaks. A total of 398 porcine faecal samples from Uttar Pradesh, Madhya Pradesh, Chhattisgarh and Jharkhand state of India were screened for porcine teschovirus (PTV), porcine sapelovirus (PSV) and enterovirus G (EV-G) by reverse transcriptase-polymerase chain reaction (RT-PCR) using 5'UTR-specific primers. The prevalence of PTV, PSV and EV-G was found to be 12.81% (51/398), 5.77% (23/398) and 24.37% (97/398), respectively. EV-G was relatively higher in circulation in Indian pigs among all the included enteric picornaviruses. Conversely, the concurrent infection of more than one enteric picornavirus was also frequent.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s13337-022-00756-0.
未标注
猪肠道微小核糖核酸病毒常导致猪腹泻和神经并发症，给养猪业造成巨大损失。本研究扩展了印度有限的猪肠道微小核糖核酸病毒数据，这对于尽早实施控制措施和防止进一步爆发是必要的。使用5'UTR特异性引物，通过逆转录聚合酶链反应（RT-PCR）对来自印度北方邦、中央邦、恰蒂斯加尔邦和贾坎德邦的398份猪粪便样本进行了猪捷申病毒（PTV）、猪萨佩洛病毒（PSV）和肠道病毒G（EV-G）的筛查。结果发现，PTV、PSV和EV-G的流行率分别为12.81%（51/398）、5.77%（23/398）和24.37%（97/398）。在所有纳入的肠道微小核糖核酸病毒中，EV-G在印度猪中的传播相对较高。相反，多种肠道微小核糖核酸病毒的同时感染也很常见。
补充信息
在线版本包含可在10.1007/s13337-022-00756-0获取的补充材料。
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