Choe Deokyeong, Jung Hyun Ho, Kim Daehwan, Shin Chul Soo, Johnston Tony Vaughn, Ku Seockmo
Fermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University Murfreesboro TN 37132 USA
Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University Seoul 03722 South Korea.
RSC Adv. 2020 Jan 8;10(3):1456-1462. doi: 10.1039/c9ra08036h. eCollection 2020 Jan 7.
The prevention and treatment of obesity using naturally derived compounds is desirable in terms of marketing and safety in the nutraceutical and functional food markets. One of the noticeable effects of pigment derivatives is the inhibition/deactivation of lipid metabolism. Our earlier studies reported that threonine (Thr), tryptophan (Trp), and 2-(-tolyl)-ethylamine (TEA) derivatives of pigment showed cholesterol-lowering, lipase-inhibitory, and adipogenic differentiation-inhibitory activities, respectively. In this work, we investigated the anti-obesity effects of a combination of Thr, Trp and TEA derivatives. C57BL/6 mice were fed a high-fat diet (HFD) and simultaneously administered one of three 1 : 1 mixtures of Thr, Trp, and TEA derivatives. After 10 weeks of feeding, the weight gains of mice fed with three combined derivatives decreased by 20.3-37.9%, compared to mice fed the HFD. The epididymal adipose tissue (EAT) weights of mice fed with the combined derivatives decreased by 42.3-60.5% compared to the HFD group, and their EAT size decreased. Transverse micro-CT imaging revealed reduction of the subcutaneous and visceral fat layers of test mice. Our results confirm that -fermented pigment derivatives have anti-obesity effects and their combinations provide a higher efficacy in the reduction of body weight and EAT weights as well as lipid accumulation in mice. The key to accomplishing high anti-obesity effect was combining Thr and Trp derivatives, which provide higher effectiveness than other combined derivatives. These observations offer a potential use of pigment derivatives as a therapeutic approach to prevention and/or treatment of obesity.
就营养保健品和功能性食品市场的营销和安全性而言，使用天然衍生化合物预防和治疗肥胖是可取的。色素衍生物的一个显著作用是抑制/失活脂质代谢。我们早期的研究报告称，色素的苏氨酸（Thr）、色氨酸（Trp）和2-（对甲苯基）乙胺（TEA）衍生物分别具有降低胆固醇、抑制脂肪酶和抑制脂肪生成分化的活性。在这项工作中，我们研究了Thr、Trp和TEA衍生物组合的抗肥胖作用。给C57BL/6小鼠喂食高脂饮食（HFD），并同时给予Thr、Trp和TEA衍生物的三种1:1混合物之一。喂食10周后，与喂食HFD的小鼠相比，喂食三种组合衍生物的小鼠体重增加减少了20.3-37.9%。与HFD组相比，喂食组合衍生物的小鼠附睾脂肪组织（EAT）重量减少了42.3-60.5%，且其EAT大小减小。横向微型CT成像显示受试小鼠的皮下和内脏脂肪层减少。我们的结果证实，发酵色素衍生物具有抗肥胖作用，其组合在减轻小鼠体重、EAT重量以及脂质积累方面具有更高的功效。实现高抗肥胖效果的关键是将Thr和Trp衍生物组合，其比其他组合衍生物具有更高的有效性。这些观察结果为色素衍生物作为预防和/或治疗肥胖的治疗方法提供了潜在用途。
