Al-Surrayai Tahani, Al-Khalaifah Hanan
Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, Safat, Kuwait.
Front Vet Sci. 2022 Apr 14;9:857294. doi: 10.3389/fvets.2022.857294. eCollection 2022.
This study investigated the impact of various concentrations of fructooligosaccharides (FOS) prebiotic on the production performance, antioxidant status, and immune response of broiler chicken. The FOS was used at 0, 0.3, 0.5, and 0.7%. The cycle included 340 broilers distributed into 4 batteries, with 85 broiler chickens in each battery. There were 5 replicates with 17 broiler chickens each, and the analyses were triplicated. The studied parameters were production performance, antioxidant status, hematological measurements, cellular and humoral immune response, intestinal acidosis, intestinal microbial counts, and volatile fatty acid (VFA) level in the hindgut. Results showed that broiler chickens fed 0.7% of FOS had significantly higher body weight gain than the control group and the groups fed 0.3% and 0.5% of FOS. Supplementing broiler feed with FOS at all levels increased the total antioxidant capacity (TAC) and reduced the malondialdehyde of the sera ( = 0.015 and 0.025, respectively). Liver catalase enzyme in the broiler chickens fed 0.5 and 0.7% of FOS was higher than that of the control group and the group fed 0.3% of FOS ( = 0.001). However, the liver MDA of the control group was higher than that of all the other groups ( = 0.031). The total WBC and heterophils % were the highest after supplementing broilers with 0.7% FOS ( = 0.004 and 0.003, respectively) at 3 wks of age. Conversely, lymphocytes and monocytes were the lowest for the 0.7% FOS group ( = 0.030 and 0.020, respectively). Dietary 0.05 and 0.7% of FOS induced the highest cellular response compared to the other treatments ( = 0.020). Thymus, bursa of Fabricious, and spleen weights were enhanced after FOS supplementation, which indicates a higher specific cellular response. To conclude, FOS prebiotic at all levels can be utilized safely to enhance the antioxidant activity and the cellular immune response of broiler chickens. Using 0.7% of FOS resulted in higher body weight of broilers. Accordingly, this amount of FOS is sufficient to reach the required results.
本研究调查了不同浓度的低聚果糖（FOS）益生元对肉鸡生产性能、抗氧化状态和免疫反应的影响。FOS的使用浓度分别为0%、0.3%、0.5%和0.7%。试验周期内，340只肉鸡被分为4个栏舍，每个栏舍有85只肉鸡。每个栏舍有5个重复，每个重复17只肉鸡，分析进行了三次。研究的参数包括生产性能、抗氧化状态、血液学指标、细胞免疫和体液免疫反应、肠道酸中毒、肠道微生物计数以及后肠挥发性脂肪酸（VFA）水平。结果显示，饲喂0.7%FOS的肉鸡体重增加显著高于对照组以及饲喂0.3%和0.5%FOS的组。在所有水平下向肉鸡饲料中添加FOS均提高了总抗氧化能力（TAC）并降低了血清丙二醛水平（分别为P = 0.015和0.025）。饲喂0.5%和0.7%FOS的肉鸡肝脏过氧化氢酶高于对照组和饲喂0.3%FOS的组（P = 0.001）。然而，对照组的肝脏丙二醛高于所有其他组（P = 0.031）。在3周龄时，向肉鸡补充0.7%FOS后，白细胞总数和嗜异性粒细胞百分比最高（分别为P = 0.004和0.003）。相反，0.7%FOS组的淋巴细胞和单核细胞最低（分别为P = 0.030和0.020）。与其他处理相比，日粮中添加0.5%和0.7%FOS诱导的细胞反应最高（P = 0.020）。补充FOS后胸腺、法氏囊和脾脏重量增加，这表明特异性细胞反应更高。总之，所有水平的FOS益生元都可安全用于提高肉鸡的抗氧化活性和细胞免疫反应。使用0.7%的FOS可使肉鸡体重更高。因此，该剂量的FOS足以达到所需效果。
