间日疟原虫一种抗原在酵母中的表达，该抗原可能用于人类疟疾疫苗。
Expression in yeast of a Plasmodium vivax antigen of potential use in a human malaria vaccine.
作者信息
Barr P J, Gibson H L, Enea V, Arnot D E, Hollingdale M R, Nussenzweig V
出版信息
J Exp Med. 1987 Apr 1;165(4):1160-71. doi: 10.1084/jem.165.4.1160.
Abstract
DNA coding for 234 amino acids of the circumsporozoite (CS) protein of Plasmodium vivax was incorporated into yeast expression vectors. The DNA encoded all the repeat domain and codons for a highly conserved sequence, KLKQP, found in CS proteins from all malaria parasites. Yeast cells transformed with these autonomously replicating plasmids expressed, upon induction, high levels of the CS polypeptide. The malaria antigen was purified in good yields from yeast extracts and was injected into mice using alum as adjuvant. The antibodies recognized the authentic CS protein, and at high dilutions, they inhibited the invasion of hepatocytes by sporozoites in vitro.
摘要
间日疟原虫环子孢子（CS）蛋白234个氨基酸的编码DNA被整合到酵母表达载体中。该DNA编码了所有的重复结构域以及在所有疟原虫的CS蛋白中都存在的一个高度保守序列KLKQP的密码子。用这些自主复制质粒转化的酵母细胞在诱导后表达出高水平的CS多肽。从酵母提取物中以高产率纯化出疟疾抗原，并使用明矾作为佐剂将其注射到小鼠体内。这些抗体识别真实的CS蛋白，并且在高稀释度下，它们在体外抑制子孢子对肝细胞的侵袭。
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