Ministry of Education, Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China.
Plant Cell Rep. 2022 Jul;41(7):1531-1548. doi: 10.1007/s00299-022-02872-z. Epub 2022 May 4.
Hydrogen sulfide positively regulates autophagy and the expression of hypoxia response-related genes under submergence to enhance the submergence tolerance of Arabidopsis. Flooding seriously endangers agricultural production, and it is quite necessary to explore the mechanism of plant response to submergence for improving crop yield. Both hydrogen sulfide (HS) and autophagy are involved in the plant response to submergence. However, the mechanisms by which HS and autophagy interact and influence submergence tolerance have not been thoroughly elucidated. Here, we reported that exogenous HS pretreatment increased the level of endogenous HS and alleviated plant cell death under submergence. And transgenic lines decreased in the level of endogenous HS, L-cysteine desulfurase 1 (des1) mutant and 35S::GFP-O-acetyl-L-serine(thiol)lyase A1 (OASA1)/des1-#56/#61, were sensitive to submergence, along with the lower transcript levels of hypoxia response genes, LOB DOMAIN 41 (LBD41) and HYPOXIA RESPONSIVE UNKNOWN PROTEIN 43 (HUP43). Submergence induced the formation of autophagosomes, and the autophagy-related (ATG) mutants (atg4a/4b, atg5, atg7) displayed sensitive phenotypes to submergence. Simultaneously, HS pretreatment repressed the autophagosome producing under normal conditions, but enhanced this process under submergence by regulating the expression of ATG genes. Moreover, the mutation of DES1 aggravated the sensitivity of des1/atg5 to submergence by reducing the formation of autophagosomes under submergence. Taken together, our results demonstrated that HS alleviated cell death through regulating autophagy and the expression of hypoxia response genes during submergence in Arabidopsis.
硫化氢通过正调控自噬和缺氧反应相关基因的表达来提高拟南芥的耐淹能力
洪水严重危及农业生产，因此有必要探索植物对水淹的响应机制，以提高作物产量。硫化氢（HS）和自噬都参与了植物对水淹的响应。然而，HS 和自噬相互作用并影响耐淹能力的机制尚未得到彻底阐明。在这里，我们报道了外源 HS 预处理可以提高内源性 HS 的水平，并减轻淹水条件下植物细胞的死亡。而内源性 HS 水平降低的转基因系、L-半胱氨酸脱硫酶 1（des1）突变体和 35S::GFP-O-乙酰-L-丝氨酸（硫）裂合酶 A1（OASA1）/des1-#56/#61 对淹水敏感，同时缺氧反应基因 LOB DOMAIN 41（LBD41）和 HYPOXIA RESPONSIVE UNKNOWN PROTEIN 43（HUP43）的转录水平较低。淹水诱导自噬体的形成，而自噬相关（ATG）突变体（atg4a/4b、atg5、atg7）对淹水表现出敏感表型。同时，HS 预处理在正常条件下抑制自噬体的形成，但通过调节 ATG 基因的表达，在淹水条件下增强了这一过程。此外，DES1 的突变通过减少淹水条件下自噬体的形成，加重了 des1/atg5 对淹水的敏感性。总之，我们的研究结果表明，HS 通过调节自噬和缺氧反应相关基因的表达来缓解拟南芥淹水过程中的细胞死亡。
