硫化氢在茉莉酸下游起作用，抑制拟南芥气孔的发育。
Hydrogen sulfide acts downstream of jasmonic acid to inhibit stomatal development in Arabidopsis.
机构信息
Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming, 650224, Yunnan, China.
College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China.
出版信息
Planta. 2020 Jan 6;251(2):42. doi: 10.1007/s00425-019-03334-9.
Main conclusion: Jasmonic acid (JA) negatively regulates stomatal development by promoting LCD expression and hydrogen sulfide (HS) biosynthesis. HS inhibits the initiation of stomata formation and acts upstream of SPEECHLESS. Abstract: Stomatal development is strictly regulated by endogenous signals and environmental cues. We recently revealed that jasmonic acid (JA) negatively regulates stomatal development in Arabidopsis thaliana cotyledons (Han et al., Plant Physiol 176:2871-2885, 2018), but the underlying molecular mechanism remains largely unknown. Here, we uncovered a role for HS in regulating stomatal development. The HS scavenger hypotaurine reversed the JA-induced repression of stomatal development in the epidermis of wild-type Arabidopsis. The HS-deficient mutant lcd displayed increased stomatal density and stomatal index values, which were rescued by treatment with sodium hydrosulfide (NaHS; an HS donor) but not JA, suggesting that JA-mediated repression of stomatal development is dependent on HS biosynthesis. The high stomatal density of JA-deficient mutants was rescued by exogenous NaHS treatment. Further analysis indicated that JA positively regulates LCD expression, L-cysteine desulfhydrases (L-CDes) activity, and endogenous HS content. Furthermore, HS represses the expression of stomate-associated genes and functions downstream of stomate-related signaling pathway components TOO MANY MOUTHS (TMM) and STOMATAL DENSITY AND DISTRIBUTION1 (SDD1) and upstream of SPEECHLESS (SPCH). Therefore, HS acts downstream of JA signaling to regulate stomatal development in Arabidopsis cotyledons.
主要结论
茉莉酸（JA）通过促进液晶（LCD）表达和硫化氢（HS）生物合成来负调控气孔发育。HS 抑制气孔形成的起始，作用于 SPEECHLESS 上游。
摘要
气孔发育受到内源性信号和环境线索的严格调控。我们最近揭示了茉莉酸（JA）负调控拟南芥子叶表皮气孔发育（Han 等人，《植物生理学》176:2871-2885，2018），但潜在的分子机制在很大程度上仍不清楚。在这里，我们发现了 HS 在调节气孔发育中的作用。HS 清除剂硫代牛磺酸逆转了 JA 对野生型拟南芥表皮气孔发育的抑制。HS 缺陷突变体 lcd 表现出增加的气孔密度和气孔指数值，用硫氢化钠（HS 的供体）处理而不是 JA 处理可挽救这些值，表明 JA 介导的气孔发育抑制依赖于 HS 的生物合成。JA 缺陷突变体的高气孔密度可通过外源 NaHS 处理得到挽救。进一步分析表明，JA 正向调节 LCD 表达、L-半胱氨酸脱硫酶（L-CDes）活性和内源性 HS 含量。此外，HS 抑制与气孔相关的基因的表达，并作用于气孔相关信号通路成分 TOO MANY MOUTHS（TMM）和 STOMATAL DENSITY AND DISTRIBUTION1（SDD1）下游和 SPEECHLESS（SPCH）上游。因此，HS 在拟南芥子叶中作为 JA 信号的下游因子来调节气孔发育。
Zotero 插件