Xploraytion, Bismarckstrasse 10-12, 10625 Berlin, Germany.
University of Lyon, INSA-Lyon, Université Claude Bernard Lyon 1, UJM-Saint Etienne, CNRS, Inserm, CREATIS UMR 5220, U1206, 69621 Lyon, France.
J Synchrotron Radiat. 2022 May 1;29(Pt 3):843-852. doi: 10.1107/S1600577522003137. Epub 2022 Apr 25.
X-ray nano-tomography with phase contrast (nanoCT) using synchrotron radiation is a powerful tool to non-destructively investigate 3D material properties at the nanoscale. In large bone lesions, such as severe bone fractures, bone cancer or other diseases, bone grafts substituting the lost bone might be necessary. Such grafts can be of biological origin or be composed of a synthetic bone substitute. The long-term functioning of artificial bone substitutes depends on many factors. Synchrotron nanoCT imaging has great potential to contribute to further the understanding of integration of implants into bone tissue by imaging the spatial interaction between bone tissue and implant, and by accessing the interface between implant material and bone tissue. With this aim, a methodology for evaluating the image quality is presented for in-line phase contrast nanoCT images of bone scaffold samples. A PMMA-embedded tricalcium phosphate scaffold was used with both a closed and an open porosity structure and bone ingrowths as a representative system of three known materials. Parameters such as spatial resolution and signal-to-noise ratio were extracted and used to explore and quantitatively compare the effects of implementation choices in the imaging setup, such as camera technology and imaging energy, on the resulting image quality. Increasing the X-ray energy from 17.5 keV to 29.6 keV leads to a notable improvement in image quality regardless of the camera technology used, with the two tested camera setups performing at a comparable level when the recorded intensity was kept constant.
使用同步辐射的 X 射线纳米断层摄影术（nanoCT）具有相位对比，是一种强大的工具，可以对纳米级的 3D 材料特性进行非破坏性研究。在大的骨病变中，例如严重的骨折、骨癌或其他疾病，可能需要用替代丢失骨的骨移植物。这些移植物可以是生物起源的，也可以是由合成骨替代物组成的。人工骨替代品的长期功能取决于许多因素。同步辐射纳米 CT 成像具有很大的潜力，可以通过对骨组织和植入物之间的空间相互作用进行成像，并通过获取植入材料和骨组织之间的界面来帮助进一步理解植入物与骨组织的整合。为此，提出了一种用于评估图像质量的方法，用于对骨支架样本的在线相位对比纳米 CT 图像进行评估。使用聚甲基丙烯酸甲酯（PMMA）嵌入的磷酸三钙（TCP）支架，具有封闭和开放的多孔结构和骨内生长，作为三种已知材料的代表性系统。提取了空间分辨率和信噪比等参数，并用于探索和定量比较成像设置中的实现选择对图像质量的影响，例如相机技术和成像能量。无论使用哪种相机技术，将 X 射线能量从 17.5keV 增加到 29.6keV 都会显著提高图像质量，当记录的强度保持不变时，两个测试的相机设置性能相当。
