Faculty of Dentistry, National University of Singapore, Singapore.
Division of Gastroenterology & Hepatology, National University Hospital, Singapore.
J Crohns Colitis. 2022 Nov 1;16(10):1628-1636. doi: 10.1093/ecco-jcc/jjac063.
Microbiome dysbiosis is associated with inflammatory destruction in Crohn's disease [CD]. Although gut microbiome dysbiosis is well established in CD, the oral microbiome is comparatively under-studied. This study aims to characterize the oral microbiome of CD patients with/without oral manifestations.
Patients with CD were recruited with age-, gender- and race-matched controls. Potential confounders such as dental caries and periodontal condition were recorded. The oral microbiome was collected using saliva samples. Microbial DNA was extracted and sequenced using shotgun sequencing. Metagenomic taxonomic and functional profiles were generated and analysed.
The study recruited 41 patients with CD and 24 healthy controls. Within the CD subjects, 39.0% had oral manifestations with the majority presenting with cobblestoning and/or oral ulcers. Principal coordinate analysis demonstrated distinct oral microbiome profiles between subjects with and without CD, with four key variables responsible for overall oral microbiome variance: [1] diagnosis of CD, [2] concomitant use of steroids, [3] concomitant use of azathioprine and 4] presence of oral ulcers. Thirty-two significant differentially abundant microbial species were identified, with the majority associated with the diagnosis of CD. A predictive model based on differences in the oral microbiome found that the oral microbiome has strong discriminatory function to distinguish subjects with and without CD [AUROC 0.84]. Functional analysis found that an increased representation of microbial enzymes [n = 5] in the butyrate pathway was positively associated with the presence of oral ulcers.
The oral microbiome can aid in the diagnosis of CD and its composition was associated with oral manifestations.
肠道微生物失调与克罗恩病（CD）的炎症破坏有关。尽管 CD 患者的肠道微生物失调已得到充分证实，但口腔微生物组的研究相对较少。本研究旨在描述有/无口腔表现的 CD 患者的口腔微生物组。
招募 CD 患者，并与年龄、性别和种族相匹配的对照者进行对照。记录潜在的混杂因素，如龋齿和牙周状况。使用唾液样本收集口腔微生物组。提取微生物 DNA 并进行测序。生成并分析宏基因组分类和功能图谱。
本研究共招募了 41 名 CD 患者和 24 名健康对照者。在 CD 患者中，39.0%有口腔表现，大多数表现为鹅卵石样改变和/或口腔溃疡。主坐标分析显示，有无 CD 的患者口腔微生物组图谱明显不同，四个关键变量负责总体口腔微生物组的差异：[1] CD 的诊断，[2] 同时使用类固醇，[3] 同时使用硫唑嘌呤，以及 4）存在口腔溃疡。鉴定出 32 种差异丰度的显著微生物物种，其中大多数与 CD 的诊断相关。基于口腔微生物组差异的预测模型发现，口腔微生物组具有很强的区分功能，可以区分有和无 CD 的患者 [AUROC 0.84]。功能分析发现，丁酸盐途径中微生物酶 [n = 5] 的代表性增加与口腔溃疡的存在呈正相关。
口腔微生物组有助于 CD 的诊断，其组成与口腔表现有关。
