Zhu Qi, Ma Zhijie, Li Haiyan, Wang Haiming, He Yaohua
Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital 600 Yishan Road Shanghai 200233 China
Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University 1954 Huashan Road Shanghai 200030 China.
RSC Adv. 2019 May 17;9(27):15582-15592. doi: 10.1039/c8ra09849b. eCollection 2019 May 14.
Rotator cuff tear (RCT) is a major challenging shoulder disease because the fibrocartilage zone is hard to regenerate in the enthesis. Electrospun membranes with aligned nanofibers can guide the ordered tissue regeneration and kartogenin (KGN) is able to stimulate chondrocyte differentiation of mesenchymal stem cells. In this study, we fabricated a functional engineered scaffold for regenerating tendon-bone enthesis in RCTs by taking advantage of both the structural guiding ability of aligned nanofibers and the biology effects of KGN. Polycaprolactone (PCL) fibrous membranes with aligned nanofibers loaded with or without KGN were fabricated using electrospinning and characterized using scanning electron microscopy (SEM). The release of KGN from PCL membranes and the effects of KGN on differentiation of mesenchymal stem cells were investigated. Results indicated that 100 μM KGN-loaded PCL (KGN-PCL) membranes significantly stimulated chondrogenic and tenogenic differentiation of rat bone marrow stromal cells. In addition, after PCL and 100 μM KGN-PCL membranes were applied to an acute rat RCT model, KGN-PCL membranes promoted fibrocartilage formation and collagen organization as well as increased cross-sectional area and load failure. In conclusion, PCL electrospun fibrous membranes with aligned nanofibers and KGN could be an effective tissue engineering scaffold to enhance tendon-bone healing in RCTs.
肩袖撕裂（RCT）是一种极具挑战性的肩部疾病，因为在肌腱附着处的纤维软骨区域很难再生。具有排列纳米纤维的电纺膜可以引导有序的组织再生，而卡托金（KGN）能够刺激间充质干细胞向软骨细胞分化。在本研究中，我们利用排列纳米纤维的结构引导能力和KGN的生物学效应，制备了一种用于在RCT中再生肌腱-骨附着处的功能性工程支架。使用静电纺丝法制备了负载或未负载KGN的具有排列纳米纤维的聚己内酯（PCL）纤维膜，并通过扫描电子显微镜（SEM）进行表征。研究了KGN从PCL膜中的释放以及KGN对间充质干细胞分化的影响。结果表明，负载100μM KGN的PCL（KGN-PCL）膜显著刺激了大鼠骨髓基质细胞的软骨生成和肌腱生成分化。此外，将PCL和100μM KGN-PCL膜应用于急性大鼠RCT模型后，KGN-PCL膜促进了纤维软骨形成和胶原组织化，以及增加了横截面积和负荷失败。总之，具有排列纳米纤维和KGN的PCL电纺纤维膜可能是一种有效的组织工程支架，可增强RCT中的肌腱-骨愈合。
