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基于聚（乙烯基己内酰胺）的具有多酶活性的温度响应性铁纳米酶

Temperature-responsive iron nanozymes based on poly(-vinylcaprolactam) with multi-enzyme activity.

作者信息

Wang Yang, Wang Wei, Gu Zhun, Miao Xiangyang, Huang Qiuyan, Chang Baisong

机构信息

Department of Medical Technology, Suzhou Chien-shiung Institute of Technology Taicang 215411 Jiangsu Province P. R. China

Nhwa Pharma. Corporation Xuzhou 221000 Jiangsu Province P. R.China.

出版信息

RSC Adv. 2020 Nov 2;10(65):39954-39966. doi: 10.1039/d0ra07226e. eCollection 2020 Oct 27.

DOI:10.1039/d0ra07226e
PMID:35515401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9057499/
Abstract

Iron (Fe)-based nanozymes are widely applied in the biomedical field due to their enzyme-like catalytic activity. Herein, Fe(ii)-based coordination polymer nanohydrogels (FeCPNGs) have been conveniently prepared as a new type of nanozyme by the chelation reaction between ferrous iron and polymer nanohydrogels. The P(VCL--NMAM) nanohydrogels prepared by a reflux precipitation polymerization method using -vinylcaprolactam (VCL) and -methylol acrylamide (NMAM) as monomers and ,-methylenebisacrylamide (MBA) as a crosslinker were esterified using PO and then chelated with Fe(ii) ions to form nanozymes with peroxidase and superoxide dismutase (SOD) activity. It was found by dynamic light scattering (DLS) and transmission electron microscopy (TEM) that the nanohydrogels prepared with a monomer concentration of 4% and mass ratio of 1 : 1 (VCL : NMAM) had more uniform particle size, better dispersion and a distinct temperature response. The results of Fourier transform infrared (FTIR), DLS, TEM, X-ray powder diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) indicated the successful preparation of the esterified nanohydrogel and FeCPNGs. Of particular importance is that such FeCPNGs can functionally mimic two antioxidant enzymes (peroxidase and superoxide dismutase) by UV analysis of catalytic oxidation between 3,3,5,5-tetramethylbenzidine (TMB) and HO and the kit analysis of SOD-like activity.

摘要

铁（Fe）基纳米酶因其类酶催化活性而在生物医学领域得到广泛应用。在此，通过亚铁离子与聚合物纳米水凝胶之间的螯合反应，简便地制备了基于Fe（II）的配位聚合物纳米水凝胶（FeCPNGs）作为一种新型纳米酶。以N-乙烯基己内酰胺（VCL）和N-羟甲基丙烯酰胺（NMAM）为单体、N,N'-亚甲基双丙烯酰胺（MBA）为交联剂，采用回流沉淀聚合法制备的P（VCL-NMAM）纳米水凝胶先用PO进行酯化，然后与Fe（II）离子螯合，形成具有过氧化物酶和超氧化物歧化酶（SOD）活性的纳米酶。通过动态光散射（DLS）和透射电子显微镜（TEM）发现，单体浓度为4%、质量比为1:1（VCL:NMAM）制备的纳米水凝胶具有更均匀的粒径、更好的分散性和明显的温度响应。傅里叶变换红外光谱（FTIR）、DLS、TEM、X射线粉末衍射（XRD）和X射线光电子能谱（XPS）结果表明酯化纳米水凝胶和FeCPNGs制备成功。特别重要的是，通过对3,3,5,5-四甲基联苯胺（TMB）与H2O2之间催化氧化的紫外分析以及类SOD活性的试剂盒分析，这种FeCPNGs可以在功能上模拟两种抗氧化酶（过氧化物酶和超氧化物歧化酶）。

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