Kaur Amritpal, Kumar Rajesh
Department of Physics, Panjab University Chandigarh 160014 India
RSC Adv. 2019 Jan 9;9(2):1095-1105. doi: 10.1039/c8ra07980c. eCollection 2019 Jan 2.
The paper presents the interaction mechanism of silver nanoparticles (AgNPs) with different antibiotics and the antibacterial efficacy of the formed conjugates. The AgNPs used in this study were synthesized from silver nitrate using sodium borohydride as a reducing agent, in the presence of PVP as a protecting agent. Two antibiotics, amikacin and vancomycin with different modes of action, were used to functionalize the synthesized PVP-capped AgNPs. The formation of antibiotic-AgNPs conjugate was confirmed by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and X-ray diffraction (XRD) and the results suggest the conjugation of both drugs to silver nanoparticle surfaces. FTIR results indicate that intermolecular hydrogen bonding exists between PVP-coated AgNPs and antibiotics. The oxygen atom coordinated with PVP was available for interaction with either amine or amide groups of drugs. Further, the antibacterial efficacy of these PVP-capped AgNPs with selected antibiotics was evaluated against and by agar well diffusion test. Synergetic bactericidal activity for antibiotic-AgNPs conjugate was observed against both microbes.
本文介绍了银纳米颗粒（AgNPs）与不同抗生素的相互作用机制以及所形成共轭物的抗菌效果。本研究中使用的AgNPs是在聚乙烯吡咯烷酮（PVP）作为保护剂的存在下，以硼氢化钠为还原剂由硝酸银合成的。两种具有不同作用方式的抗生素，阿米卡星和万古霉素，被用于使合成的PVP包覆的AgNPs功能化。通过紫外可见光谱、傅里叶变换红外光谱（FTIR）、动态光散射（DLS）和X射线衍射（XRD）证实了抗生素 - AgNPs共轭物的形成，结果表明两种药物均与银纳米颗粒表面共轭。FTIR结果表明，PVP包覆的AgNPs与抗生素之间存在分子间氢键。与PVP配位的氧原子可用于与药物的胺基或酰胺基相互作用。此外，通过琼脂孔扩散试验评估了这些PVP包覆的AgNPs与选定抗生素对[具体微生物1]和[具体微生物2]的抗菌效果。观察到抗生素 - AgNPs共轭物对两种微生物均具有协同杀菌活性。
