Li Biyun, Xiong Feng, Yao Bo, Du Qian, Cao Jun, Qu Jiangang, Feng Wei, Yuan Huihua
School of Life Sciences, Nantong University Nantong Jiangsu 226019 China
School of Textile and Clothing, Nantong University Nantong Jiangsu 226019 China.
RSC Adv. 2020 May 15;10(32):18614-18623. doi: 10.1039/d0ra03224g. eCollection 2020 May 14.
Electrospun poly(l)-lactide (PLLA) ultrafine fibers are a biodegradable and biocompatible scaffold, widely used in tissue engineering applications. Unfortunately, these scaffolds have some limitations related to the absence of bioactivity and antibacterial capacity. In this study, dopamine-functionalized reduced graphene oxide (rGO)/PLLA composite nanofibers were fabricated electrospinning. The morphology and the physicochemical and biological properties of the composite nanofibers were investigated. The results indicate that incorporating rGO improves the hydrophilic, mechanical, and biocompatibility properties of PLLA nanofibers. Tetracycline hydrochloride (TC)-loaded rGO/PLLA composite nanofibers showed better controlled drug release profiles compared to GO/PLLA and PLLA nanofibrous scaffolds. Drug-loaded nanofibrous scaffolds showed significantly improved antibacterial activity against Gram-negative () and Gram-positive (). Additionally, rGO/PLLA composite nanofibers exhibited enhanced cytocompatibility. Thus, it can be concluded that rGO/PLLA composite nanofibers allow the development of multifunctional scaffolds for use in biomedical applications.
静电纺聚左旋乳酸（PLLA）超细纤维是一种可生物降解且具有生物相容性的支架材料，广泛应用于组织工程领域。遗憾的是，这些支架存在一些局限性，如缺乏生物活性和抗菌能力。在本研究中，通过静电纺丝制备了多巴胺功能化的还原氧化石墨烯（rGO）/PLLA复合纳米纤维。对复合纳米纤维的形态、物理化学和生物学性质进行了研究。结果表明，掺入rGO可改善PLLA纳米纤维的亲水性、机械性能和生物相容性。与氧化石墨烯/聚左旋乳酸（GO/PLLA）和PLLA纳米纤维支架相比，载有盐酸四环素（TC）的rGO/PLLA复合纳米纤维显示出更好的药物控释特性。载药纳米纤维支架对革兰氏阴性菌（）和革兰氏阳性菌（）表现出显著增强的抗菌活性。此外，rGO/PLLA复合纳米纤维表现出增强的细胞相容性。因此，可以得出结论，rGO/PLLA复合纳米纤维可用于开发用于生物医学应用的多功能支架。
