Xu Weijian, Sun Yingjia, Wang Jia, Wang Baixiang, Xu Fanxing, Xie Zhijian, Wang Yu
Department of Oral and Maxillofacial Surgery, Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University Hangzhou 310006 China.
Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine 166 Qiutaobei Road, Shangcheng District Hangzhou Zhejiang 310016 China.
RSC Adv. 2022 May 3;12(21):13192-13202. doi: 10.1039/d2ra00498d. eCollection 2022 Apr 28.
A dry socket is one of the most common complications after tooth extraction. The main etiologies are the loss of blood clots in the socket and the inflammation reaction caused by infection. Current studies on how to prevent dry sockets could not solve these two etiologies at the same time. Recent studies have demonstrated the anti-inflammation role of silibinin. In this study, silibinin was engineered into GelMA hydrogels (Sil-GelMA) with a concentration of 30 mM. The surface characteristics were observed by scanning electron microscopy and the successful loading of silibinin was detected by FTIR spectrometry. The Sil-GelMA hydrogels presented the sustained release ability of silibinin and slow degradation performance of GelMA. Furthermore, silibinin inhibited the inflammatory reaction by inducing M2-type macrophage polarization, promoting the secretion of anti-inflammatory factors (CD206, IL-10) and inhibiting the secretion of anti-inflammatory factors (IL-1β, iNOS). Silibinin also increased the secretion of vascularization-related factor VEGF and promoted vascularization . This study suggested that the Sil-GelMA hydrogels not only had an anti-inflammatory effect, but also had the potential to promote vascularization. Based on these results, the Sil-GelMA hydrogels might provide a promising prospect for prevention of dry sockets in the future.
干槽症是拔牙后最常见的并发症之一。主要病因是牙槽窝内血凝块的丢失以及感染引起的炎症反应。目前关于如何预防干槽症的研究无法同时解决这两个病因。最近的研究表明水飞蓟宾具有抗炎作用。在本研究中，将水飞蓟宾制成浓度为30 mM的甲基丙烯酰化明胶水凝胶（Sil-GelMA）。通过扫描电子显微镜观察表面特征，并用傅里叶变换红外光谱法检测水飞蓟宾的成功负载。Sil-GelMA水凝胶呈现出水飞蓟宾的缓释能力和明胶水凝胶的缓慢降解性能。此外，水飞蓟宾通过诱导M2型巨噬细胞极化、促进抗炎因子（CD206、IL-10）的分泌以及抑制促炎因子（IL-1β、iNOS）的分泌来抑制炎症反应。水飞蓟宾还增加了血管生成相关因子VEGF的分泌并促进血管生成。本研究表明，Sil-GelMA水凝胶不仅具有抗炎作用，还具有促进血管生成的潜力。基于这些结果，Sil-GelMA水凝胶可能为未来预防干槽症提供一个有前景的方案。
