采用高速逆流色谱、基于亲和的超滤和液相色谱-串联质谱联用技术对根茎中的酪氨酸酶抑制剂进行表征
Characterization of Tyrosinase Inhibitors in Rhizome Using a Combination of High-Speed Counter-Current Chromatography, Affinity-Based Ultrafiltration, and Liquid Chromatography-Tandem Mass Spectrometry.
作者信息
Wang Zhiqiang, Wang Ning, Han Dandan, Yan Hongyuan
机构信息
Key Laboratory of Public Health Safety of Hebei Province, School of Public Health, Hebei University, Baoding, China.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Pharmaceutical Sciences, Hebei University, Baoding, China.
出版信息
Front Nutr. 2022 Apr 18;9:862773. doi: 10.3389/fnut.2022.862773. eCollection 2022.
Dryopteris crassirhizoma rhizome (DCR) inhibits melanin production in B16F10 melanoma cells and tyrosinase activity. The melanin content and tyrosinase activity of DCR-treated zebrafish embryos were determined to evaluate the inhibitory effect of DCR on melanogenesis. Moreover, an off-line hyphenated method combining the high-speed counter-current chromatography, affinity-based ultrafiltration, and liquid chromatography-tandem mass spectrometry was used to identify and characterize the DCR compounds with tyrosinase inhibitory activity. Our results indicated that DCR significantly decreased the melanin content and tyrosinase activity in zebrafish embryos in a dose-dependent manner; moreover, 22 compounds in DCR presented tyrosinase inhibitory activity. molecular docking prediction data indicated that the 22 compounds in DCR can form stable conformations in the active site pocket of tyrosinase.
粗茎鳞毛蕨根茎（DCR）可抑制B16F10黑色素瘤细胞中的黑色素生成及酪氨酸酶活性。测定了经DCR处理的斑马鱼胚胎的黑色素含量和酪氨酸酶活性，以评估DCR对黑色素生成的抑制作用。此外，采用高速逆流色谱、基于亲和的超滤和液相色谱-串联质谱联用的离线联用方法，对具有酪氨酸酶抑制活性的DCR化合物进行鉴定和表征。我们的结果表明，DCR以剂量依赖的方式显著降低了斑马鱼胚胎中的黑色素含量和酪氨酸酶活性；此外，DCR中的22种化合物具有酪氨酸酶抑制活性。分子对接预测数据表明，DCR中的22种化合物可在酪氨酸酶的活性位点口袋中形成稳定构象。
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