University of Sarajevo-Faculty of Pharmacy, Zmaja od Bosne 8, 71 000, Sarajevo, Bosnia and Herzegovina.
University of Novi Sad-Faculty of Medicine, Hajduk Veljkova 3, 21000, Novi Sad, Serbia.
Sci Rep. 2022 May 6;12(1):7470. doi: 10.1038/s41598-022-11171-2.
Plant-derived products are frequently found as ingredients in cosmetics. However, the current data show non-neglectable skin sensitizing potential of these preparations suggesting an urgent need for data regarding their health safety profile. The aim of this study was to assess the skin sensitization potential of commercial essential oils by selected Lamiaceae species (Lavandula angustifolia, Melissa officinalis, Mentha longifolia, Thymus vulgaris, Salvia officinalis, and Rosmarinus officinalis) using a chemistry-based Direct Peptide Reactivity Assay (DPRA) in order to predict their potential allergic properties. In the DPRA assay, nucleophile-containing synthetic peptides (cysteine peptide and lysine peptide) were incubated with the test substance for 24 h. Depletion of the peptide in the reaction mixture was measured by high-pressure liquid chromatography (HPLC) using UV detection and the average peptide depletion data for cysteine and lysine was then calculated. Menthae longifoliae aetheroleum showed no or minimal reactivity with 4.48% cysteine depletion, Rosmarini aetheroleum and Salviae aetheroleum showed low reactivity with the 12.79% and 15.34% of cysteine depletion, respectively, while the other analyzed essential oils showed moderate reactivity with the cysteine depletion between 23.21 and 48.43%. According to DPRA predictive analysis, only Menthae longifoliae aetheroleum can be classified as negative, while all other essential oils may be classified as positive, thus having the potential to cause skin sensitization.
植物衍生产品经常作为化妆品的成分。然而，目前的数据显示这些制剂具有不可忽视的皮肤致敏潜力，这表明迫切需要有关其健康安全状况的数据。本研究旨在使用基于化学的直接肽反应性测定法 (DPRA) 评估所选唇形科植物（狭叶薰衣草、药用迷迭香、薄荷醇、普通百里香、药用鼠尾草和迷迭香）的商业精油的皮肤致敏潜力，以预测其潜在的过敏特性。在 DPRA 测定中，亲核含有的合成肽（半胱氨酸肽和赖氨酸肽）与测试物质孵育 24 小时。通过使用紫外检测的高压液相色谱法（HPLC）测量反应混合物中肽的消耗，并计算半胱氨酸和赖氨酸的平均肽消耗数据。薄荷醇精油表现出无或最小的反应性，半胱氨酸消耗率为 4.48%，迷迭香油和鼠尾草精油表现出低反应性，半胱氨酸消耗率分别为 12.79%和 15.34%，而其他分析的精油则表现出中等反应性，半胱氨酸消耗率在 23.21%至 48.43%之间。根据 DPRA 预测分析，只有薄荷醇精油可被归类为阴性，而其他所有精油可能被归类为阳性，因此具有引起皮肤致敏的潜力。
